Edamame's (Glycine max L. Merrill) consumption in the US has also been growing due to purported health benefits. Edamame grows well around the US, but few have measured the growth and yield in the mid-Atlantic region. The objective of these studies were to determine the potential yield of edamame, determine how yield components change with planting date and cultivar, and to measure total protein, lipids, antioxidant activity and isoflavone concentrations at harvest.. The five cultivars (BeSweet 292, BeSweet 2015, BeSweet 2001, Midori Giant and Sunrise) used in the cultivar evaluation trial and for nutritional constituents analysis were grown in Painter, Virginia in 2008 and 2009. The cultivar evaluation trial yielded between 5,600 and 8,400 kg per ha. Percent marketable pods ranged from 74.3 and 85.6% in the cultivar evaluation trial. There were significant differences in average seed size across cultivars in both years. Cultivar lipid content followed the same patterns in both years with 2009 having lower overall concentrations than 2008. Protein contents were similar in 2008 and 2009. Both years, "BeSweet 2015" and "BeSweet 2001" had high radical scavenging ability and Midori Giant had the lowest. In 2008, there were no significant differences in the ORAC assay. "BeSweet 292" had significantly more reducing activity in the DPPH assay than Sunrise in 2009. Isoflavones were measured in 2008 and 2009 at harvest and temporally in 2009. Of all isoflavones, Malonyl genistin had the highest concentration. Edamame is a suitable crop for cultivation in Virginia, and is high in nutritional quality.