Vibrio spp. disinfection and immunization of Cobia (Rachycentron canadum) for the prevention of disease in aquaculture facilities
Machen, John Wesley
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Cobia (Rachycentron canadum) is a tropical marine fish, with increasing commercial aquaculture importance worldwide. One of the major limitations to intensive aquaculture is disease. Diseases spread rapidly in an aquatic environment and pose a major threat to the development and introduction of new species, such as cobia, in aquaculture. This is due to the necessity to use wild caught broodstock, which pose a greater threat to introducing disease to a facility. Bacteria of the genus Vibrio play a major role in the diseases of cultured cobia and other species of marine fish. The goal of this study is to reduce the incidence of disease in a population, by either eliminating the potential pathogen or increasing the resistance of the host. To reach that goal, a disinfection assay to evaluate the effectiveness of nine common aquaculture chemical disinfecting compounds was evaluated against two bacterial pathogens (Vibrio anguillarum and V. ordalii). Both bacterial species were susceptible to a variety of common disinfecting compounds including Chloramine-TÂ®, chlorine, ethanol, iodine, LysolÂ®, RoccalÂ®-D Plus, and Virkon-SÂ®. In addition, both species showed a resistance to disinfection with formalin and tap water. The humoral immune response of cobia to vaccination with a commercially-available vaccine for Vibrio spp. was evaluated by an ELISA. There was a significant difference between control and vaccinated groups (P<0.0001), showing significant antibody production resulting from vaccination.
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