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dc.contributor.authorVoigt, Elizabeth Elenaen_US
dc.date.accessioned2014-03-14T20:41:39Z
dc.date.available2014-03-14T20:41:39Z
dc.date.issued2010-07-14en_US
dc.identifier.otheretd-07192010-120918en_US
dc.identifier.urihttp://hdl.handle.net/10919/34066
dc.description.abstractAn in vitro arterial flow bioreactor system for the generation of physiological flows in a biological environment was designed, constructed, and characterized. The design was based on models previously used to investigate the response of endothelial cells to shear. The model interfaces a bioreactor with flow elements to compose a flow loop that reproduces arterial flow conditions within the bioreactor. High-resolution (8.6 microns) time-resolved (4 ms) velocity field measurements within the bioreactor were obtained using Particle Image Velocimetry (PIV). Two physiological flows were considered, corresponding to medium human arteries at rest and exercise conditions: first, with an average Reynolds number of 150 and a Womersley parameter of 6.4, and second, with an average Reynolds number of 300 and a Womersley parameter of 9.0. Two cases were considered: first, using a smooth artery section, and second, with a confluent layer of human microvascular endothelial cells grown on the inner surface of the artery section. The instantaneous wall shear stress, time-averaged wall shear stress, and oscillatory shear index were computed from the velocity field measurements and compared for the cases with and without cells. These measurements were used to assess the value of the system for measurement of correlations between fluid dynamics and the response of biological tissue. It was determined that the flow present in such a system is not an accurate reproduction of physiological flow, and that direct measurement of the flow is necessary for accurate quantification of cellular response to fluid parameters.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartVoigt_EE_T_2010_Copyright.pdfen_US
dc.relation.haspartVoigt_EE_T_2010.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectPIVen_US
dc.subjectbioreactoren_US
dc.subjectarterial flowen_US
dc.subjectwall shear stressen_US
dc.titleHydrodynamic Characterization of an Arterial Flow Bioreactoren_US
dc.typeThesisen_US
dc.contributor.departmentMechanical Engineeringen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineMechanical Engineeringen_US
dc.contributor.committeememberTropea, Cameron D.en_US
dc.contributor.committeememberPelz, Peter F.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07192010-120918/en_US
dc.contributor.committeecochairRylander, Marissa Nicoleen_US
dc.contributor.committeecochairVlachos, Pavlos P.en_US
dc.date.sdate2010-07-19en_US
dc.date.rdate2010-08-19
dc.date.adate2010-08-19en_US


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