Baculovirus-directed expression of the phosphorylase kinase catalytic subunit :pseudosubstrate and calmodulin regulation
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Phosphorylase kinase (EC 220.127.116.11) is a key enzyme involved in the regulation of the glycogenolysis pathway. It catalyzes the Ca2+ -dependent phosphorylation and activation of the enzyme glycogen phosphorylase to make the active form glycogen phosphorylase. Phosphorylase kinase is composed of 4 subunits with a stoichiometry of (Î±Î²Î³Î´)4. The Î³ subunit is the catalytic subunit. The regulatory domain (residues 277-387) of Î³ contains a sequence resembling the sites phosphorylated in known Î³ substrates with the exception that a valine332 occurs at the analogous position of the phosphorylated serine or threonine residue.
- Doctoral Dissertations