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dc.contributor.authorSpellerberg, Amy Marieen_US
dc.date.accessioned2014-03-14T21:39:29Z
dc.date.available2014-03-14T21:39:29Z
dc.date.issued2003-04-11en_US
dc.identifier.otheretd-07042003-191338en_US
dc.identifier.urihttp://hdl.handle.net/10919/43527
dc.description.abstractImprovements in turkey reproductive efficiency is a very desirable goal for the turkey industry. The ability to maintain turkey hens in the egg-laying (LAY) stage and produce one additional egg per hen a year is estimated to save the turkey industry approximately $1.5 million dollars per year. Overall protein expression generated by tissues of the hypothalamic-hypophyseal complex, namely the anterior pituitary, of the mature turkey hen have a profound impact on reproductive cycling (Scanes, 2000). One of the key physiological factors produced by the anterior pituitary and shown to play a significant role in the regulation of egg laying is the protein prolactin (Prl). The objectives for this study are to examine the overall protein expression patterns from turkey hen pituitary tissue during the nonphotostimulated (NPS), photostimulated (PS), and egg laying (LAY) stages. Attempts to isolate transcription factors that regulate the expression of Prl using an affinity chromatography technique or southwestern screening of a bacteriophage expression library were not successful. A global analysis of protein expression, using two-dimensional polyacrylamide gels (2D gels), was conducted using whole cell, cytoplasmic and nuclear protein extracts from pituitary tissue collected during the NPS, PS and LAY reproductive stages. Approximately 1,046 proteins ranging in pI from 4.6-8.2 and molecular weights between 100 kDa-6kDa were resolved. Protein expression patterns were replicated and verified using pituitaries harvested from NPS, PS and LAY stage turkey hens from another laboratory. Proteins showing considerable changes (563 proteins increased in expression and 98 proteins decreased in expression from the NPS to the LAY stage) in their expression between the reproductive stages were grouped in analysis sets for future identification. These proteins may prove to be important to the reproductive cycling of the turkey hen and warrant future investigation. The results of this study contribute to the overall understanding of the role that the pituitary, as a critical part of the hypothalamic-hypophyseal complex, plays in turkey hen reproductive cycling.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartFINALTHESIS.5.9.04.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectprotein expressionen_US
dc.subjectturkeyen_US
dc.subjectpituitaryen_US
dc.subjectreproductive cycleen_US
dc.titleCharacterization of Pituitary Protein Expression Patterns During Stages in the Reproductive Cycle of Turkey Hensen_US
dc.typeThesisen_US
dc.contributor.departmentAnimal and Poultry Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineAnimal and Poultry Sciencesen_US
dc.contributor.committeechairWong, Eric A.en_US
dc.contributor.committeememberDenbow, Donald Michaelen_US
dc.contributor.committeememberRutherford, Charles L.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07042003-191338/en_US
dc.date.sdate2003-07-04en_US
dc.date.rdate2004-07-20
dc.date.adate2004-07-20en_US


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