Characterization and optimization of supercritical fluid chromatography with on-line Fourier transform infrared detection
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The interface of a Fourier Transform Infrared (FTIR) spectrometer to a packed column (analytical scale) SFC is demonstrated. Characterization of mobile phases for SFC-FTIR is presented. Also, parameters for the optimization of spectral sensitivity, such as mobile phase transparency and flow cell design, are discussed. Demonstrations are made via model mixture separations.
Finally, the application of this technique to the analysis of free fatty acids is presented. Identification of carboxylic acids of carbon number 6-18 is made on several standard mixtures and on free fatty acids extracted from various complex samples. The spectral identification of the compounds as free fatty acids is presented, and spectral evidence for dimerization of the acids in CO 2 and the coelution of saturated and unsaturated carboxylic acids is shown.
- Masters Theses