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dc.contributor.authorGoehring, Lutz Steffenen_US
dc.date.accessioned2014-03-14T21:47:28Z
dc.date.available2014-03-14T21:47:28Z
dc.date.issued1998-08-14en_US
dc.identifier.otheretd-101098-101319en_US
dc.identifier.urihttp://hdl.handle.net/10919/45145
dc.description.abstractEquine Protozoal Myeloencephalitis is the most frequently diagnosed neurologic disorder of horses in the united states, which is caused by the protozoan organism Sarcocystis neurona. The disease has a profound impact on the American Horse Industry. This impact includes prolonged and expensive treatment without a guaranteed return to a previous level of use for the individual horse. Poor respponse to and prolonged duration of treatment may suggest an immune mediated impariement of host response. There is limited information about the direct interaction between the pathogen and the host. In two in vitro experiments we investigated a) whether the presence of the protozoan organism can influence mitogen-stimulated peripheral blood mononuclear cells (PBMCs), suggesting a direct influence of the protozoan organism on cells of the immune system, and b) if cerebrospinal fluid (CSF) from horses with EPM has an effect on mitogen-stimulated PBMCs, suggesting that the microenvironment of the site of infection influences the course of disease. Experiment 1: Mitogen simulated PBMCs from EPM affected and control horses were co-cultured with fragments of freeze thawed bovine turbinate cells that were infected with S. neurona merozoites. Compared to controls PBMCs co-cultured with S. neurona fragments were the only cells that showed a decreased proliferation (p<0.05). A difference between EPM affected and control horses could not be detected (p>0.05). These results may imply that the persistence of S. neurona infection in the horses CNS is, in part, due to a pathogen-derived mechanism that attentuates the hosts immune response. Experiment 2: Mitogen stimulated PBMCs from a horse affected with EPM and a control were co-cultured n the presence of CSF from EPM affected and uninfected controls. Prior to co-culture the CSF was fractionated by a filtration process over two microfilter units. An identical volume of NaCl (0.9%) served as a control for the volume of CSF that was added. The proliferation assay revealed a deviation of the response depending on cell donor and CSF fraction used. The effect was independant of the protein concentration of the CSF fraction, and a decrease in lymphocyte proliferation was not caused by increased cellular death. This suggests the presence of subsets within the CSF which have a stimulatory of suppressive influence on the cells in culture. The effect was cell donor dependant which implies a difference in lymphocyte subsets between the two horses that were used.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartThesisF2.pdfen_US
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectSarcocystis neuronaen_US
dc.subjectEquine Protozoal Myeloencephalitisen_US
dc.subjectimmune priviledged siteen_US
dc.subjectLymphocyte proliferation assayen_US
dc.subjectHorseen_US
dc.titleEquine Protozoal Myeloencephalitis. Preliminary Investigation of Protozoan-Host interactions in the horseen_US
dc.typeThesisen_US
dc.contributor.departmentVeterinary Medical Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineVeterinary Medical Sciencesen_US
dc.contributor.committeechairFurr, Martin O.en_US
dc.contributor.committeememberBuechner-Maxwell, Virginia A.en_US
dc.contributor.committeememberAhmed, S. Ansaren_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-101098-101319/en_US
dc.date.sdate1998-10-11en_US
dc.date.rdate1999-04-11
dc.date.adate1998-04-11en_US


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