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dc.contributorVirginia Techen_US
dc.contributor.authorSalmanzadeh, Alirezaen_US
dc.contributor.authorKittur, Harshaen_US
dc.contributor.authorSano, Michael B.en_US
dc.contributor.authorRoberts, Paul C.en_US
dc.contributor.authorSchmelz, Eva M.en_US
dc.contributor.authorDavalos, Rafael V.en_US
dc.date.accessioned2014-05-14T13:35:38Z
dc.date.available2014-05-14T13:35:38Z
dc.date.issued2012-06-01
dc.identifier.citationSalmanzadeh, A.; Kittur, H.; Sano, M. B.; Roberts, P. C.; Schmelz, E. M.; Davalos, R. V., "Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis," Biomicrofluidics 6, 024104 (2012); http://dx.doi.org/10.1063/1.3699973
dc.identifier.issn1932-1058
dc.identifier.urihttp://hdl.handle.net/10919/47982
dc.description.abstractOvarian cancer is the leading cause of death from gynecological malignancies in women. The primary challenge is the detection of the cancer at an early stage, since this drastically increases the survival rate. In this study we investigated the dielectrophoretic responses of progressive stages of mouse ovarian surface epithelial (MOSE) cells, as well as mouse fibroblast and macrophage cell lines, utilizing contactless dielectrophoresis (cDEP). cDEP is a relatively new cell manipulation technique that has addressed some of the challenges of conventional dielectrophoretic methods. To evaluate our microfluidic device performance, we computationally studied the effects of altering various geometrical parameters, such as the size and arrangement of insulating structures, on dielectrophoretic and drag forces. We found that the trapping voltage of MOSE cells increases as the cells progress from a non-tumorigenic, benign cell to a tumorigenic, malignant phenotype. Additionally, all MOSE cells display unique behavior compared to fibroblasts and macrophages, representing normal and inflammatory cells found in the peritoneal fluid. Based on these findings, we predict that cDEP can be utilized for isolation of ovarian cancer cells from peritoneal fluid as an early cancer detection tool. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3699973] Actual pdf downloaded from NCBI.
dc.description.sponsorshipNational Science Foundation EFRI 0938047
dc.description.sponsorshipVirginia Tech Institute for Critical Technology and Applied Science (ICTAS)
dc.description.sponsorshipDiversity Summer Research Program (DSRP)
dc.description.sponsorshipNIH RO1 CA118846
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_US
dc.publisherAmerican Institute of Physics
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectForce microscopyen_US
dc.subjectBreast canceren_US
dc.subjectDielectricsen_US
dc.subjectStem-cellsen_US
dc.subjectSeparationen_US
dc.subjectBlooden_US
dc.subjectElectrorotationen_US
dc.subjectBiomarkersen_US
dc.subjectFrequencyen_US
dc.subjectMembranesen_US
dc.subjectBiochemical research methodsen_US
dc.subjectBiophysicsen_US
dc.subjectNanoscience & nanotechnologyen_US
dc.subjectPhysics, fluids & plasmasen_US
dc.titleDielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis
dc.typeArticle - Refereed
dc.contributor.departmentBiomedical Engineering and Mechanicsen_US
dc.contributor.departmentBiomedical Sciences and Pathobiologyen_US
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen_US
dc.contributor.departmentInstitute for Critical Technology and Applied Science (ICTAS)en_US
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen_US
dc.identifier.urlhttp://scitation.aip.org/content/aip/journal/bmf/6/2/10.1063/1.3699973
dc.date.accessed2014-05-09
dc.title.serialBiomicrofluidics
dc.identifier.doihttps://doi.org/10.1063/1.3699973
dc.type.dcmitypeText


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