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dc.contributorVirginia Tech
dc.contributor.authorKniel, K. E.
dc.contributor.authorSumner, Susan S.
dc.contributor.authorPierson, M. D.
dc.contributor.authorZajac, A. M.
dc.contributor.authorHackney, C. R.
dc.contributor.authorFayer, R.
dc.contributor.authorLindsay, D. S.
dc.date.accessioned2014-06-20T14:13:12Z
dc.date.available2014-06-20T14:13:12Z
dc.date.issued2004-08
dc.identifier.citationK. E. Kniel, S. S. Sumner, M. D. Pierson, A. M. Zajac, C. R. Hackney, R. Fayer, and D. S. Lindsay (2004). 'Effect of Hydrogen Peroxide and Other Protease Inhibitors on Cryptosporidium parvum Excystation and In Vitro Development," Journal of Parasitology, Vol. 90, No. 4, pp. 885-888. doi: http://dx.doi.org/10.1645/GE-203R1
dc.identifier.issn0022-3395
dc.identifier.urihttp://hdl.handle.net/10919/49038
dc.description.abstractThis study was undertaken to observe the effects of hydrogen peroxide on Cryptosporidium parvum oocysts with respect to protease activity in comparison to known protease inhibitors. In assessing the possible mechanisms of action of hydrogen peroxide, treatment effectiveness was analyzed using 3 assays and the potential roles of proteases and cations were considered. Treatment of C. parvum oocysts with hydrogen peroxide inhibited protease activity up to 50% compared with untreated controls. Treatment of oocysts with chemicals that affect sulfhydryls, including N-ethylmaleimide and dithiolthreitol, inhibited protease activity by > 90%. Treatment of oocysts with these chemicals, along with the protease inhibitors, phenylmethylsulfonyl fluoride (PMSF), ethylenediamine-tetraacetic acid, and cystatin, inhibited protease activity as well as in vitro excystation and infection in a cell culture assay. Several mechanisms may result in the successful inhibition of infection and excystation by hydrogen peroxide treatment, including: oxidation of oocyst wall proteins or lipids, chelating of cations necessary for infection, or hydroxyl radical-induced DNA damage to sporozoites, or both.
dc.description.sponsorshipCSREES USDA Special Food Safety Grant 98-34382-6916
dc.description.sponsorshipVPI&SU HATCH project 135563
dc.description.sponsorshipNew Initiative Grant from the Virginia-Maryland Regional College of Veterinary Medicine
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_US
dc.publisherAmerican Society of Parasitology
dc.subjectproteolytic activity
dc.subjectproteinase activity
dc.subjectserine-protease
dc.subjectoocysts
dc.subjectsporozoites
dc.subjectcells
dc.subjectinvasion
dc.subjectidentification
dc.subjectinactivation
dc.subjectmerozoites
dc.subjectparasitology
dc.titleEffect of hydrogen peroxide and other protease inhibitors on Cryptosporidium parvum excystation and in vitro development
dc.typeArticle - Refereed
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-203R1
dc.date.accessed2014-06-16
dc.title.serialJournal of Parasitology
dc.identifier.doihttps://doi.org/10.1645/ge-203r1
dc.type.dcmitypeText


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