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dc.contributor.authorGibson, Bethany Galeen_US
dc.date.accessioned2014-07-31T08:00:09Z
dc.date.available2014-07-31T08:00:09Z
dc.date.issued2014-07-30en_US
dc.identifier.othervt_gsexam:3446en_US
dc.identifier.urihttp://hdl.handle.net/10919/49694
dc.description.abstractPrevious research in bovine embryology has found that embryos cultured individually have limited ability to develop compared to their counterparts cultured in a group of other embryos. This investigation aimed to find if any of three different interventions over two experiments would increase development of individually cultured embryos to that of group cultured embryos. In the first experiment both the addition of serum/serum replacer and a co-culture with bovine granulosa cells were applied to individually cultured embryos in a 3x2 design. None of the interventions was found to be significantly different from the others, and all resulted in significantly lower development than embryos cultured as a group (avg. 4.7 +/- 1.93% individual vs. 21.7 +/- 3.76% group). However, a significant difference was found in the hatching rate between blastocysts cultured in media including cells (71.4 +/- 17.07%) and those cultured without cells (18.1 +/- 11.63%). In the second experiment, embryos were either cultured in standard droplets or microwells made at the bottom of culture droplets either in groups or individually for a 2x2 design. This experiment experienced poor development in all treatments including the group control, and none of the treatments were found to be significantly different from each other. However, the hatching rate of blastocysts cultured in multiple microwells was significantly higher than those cultured individually in droplets. To summarize, none of the treatments increased the development rate, but embryos cultured with granulosa cell co-cultures and in group microwells showed improvements in hatching rates.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsThis Item is protected by copyright and/or related rights. Some uses of this Item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en_US
dc.subjectbovineen_US
dc.subjectembryoen_US
dc.subjectindividual cultureen_US
dc.titleImproving the development of bovine in vitro produced embryos cultured individuallyen_US
dc.typeThesisen_US
dc.contributor.departmentVeterinary Medicineen_US
dc.description.degreeMSen_US
thesis.degree.nameMSen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineBiomedical and Veterinary Sciencesen_US
dc.contributor.committeechairClark-Deener, Sherrie Gayleen_US
dc.contributor.committeememberEyestone, Willard Hen_US
dc.contributor.committeememberEaly, Alan Daleen_US


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