Show simple item record

dc.contributor.authorDove, Chris Haysen
dc.date.accessioned2014-08-13T14:40:19Zen
dc.date.available2014-08-13T14:40:19Zen
dc.date.issued1985en
dc.identifier.urihttp://hdl.handle.net/10919/50088en
dc.description.abstractRibosomal 5.85 RNA is a component of the large (605) ribosomal subunit in eucaryotes. Studies of 5.85 rRNA in solution have shown that under certain conditions, including standard isolation procedures, the molecule complexes with itself to form dimers and higher multimers. Two models have been proposed in the literature to explain the intermolecular interactions responsible for 5.85 rRNA dimer formation. The terminal interaction model of Sitz et al. (Biochem. 17, 5811-5815, 1978) proposes that the dimer forms through base-pairing of the 5' and 3' terminal sequences of two 5.85 rRNA molecules. Pavlakis et al. (Nucl. Acids Res. 7, 2213-2237, 1978) showed that 5.85 rRNA lacking the 3' terminal region was capable of forming dimer. They proposed an alternative model for 5.85 rRNA dimer formation in which an entirely different part of the molecule interacts to form a double-stranded palindrome. In this study, enzymatic probing techniques and stability measurements (both experimental and theoretical) were used to determine which model most accurately describes the intermolecular interactions of the 5.88 rRNA dimer. The methods used for determining stability were not able to discriminate between the models for 5.88 rRNA dimer formation. Results from the structural probing studies, however, support the terminal interaction model and indicate that the palindrome interaction does not occur in 5.88 rRNA dimer formed from the intact molecule.en
dc.format.extentvii, 89 leavesen
dc.format.mimetypeapplication/pdfen
dc.publisherVirginia Polytechnic Institute and State Universityen
dc.relation.isformatofOCLC# 12939423en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1985.D683en
dc.subject.lcshRNAen
dc.subject.lcshRibosomesen
dc.titleModels for the formation of 5.8S ribosomal RNA dimeren
dc.typeThesisen
dc.contributor.departmentBiochemistry and Nutritionen
dc.description.degreeMaster of Scienceen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelmastersen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.disciplineBiochemistry and Nutritionen
dc.type.dcmitypeTexten
dc.description.adminincomplete_metadataen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record