Virginia Tech
    • Log in
    View Item 
    •   VTechWorks Home
    • ETDs: Virginia Tech Electronic Theses and Dissertations
    • Masters Theses
    • View Item
    •   VTechWorks Home
    • ETDs: Virginia Tech Electronic Theses and Dissertations
    • Masters Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Seminal plasma and freeze-thaw injury to bovine sperm

    Thumbnail
    View/Open
    LD5655.V855_1972.G47.pdf (1.234Mb)
    Downloads: 94
    Date
    1972
    Author
    Gerber, Lawrence E.
    Metadata
    Show full item record
    Abstract
    Using a split-ejaculate technique, two experiments were conducted to determine the influence of sperm exposure to seminal plasma on freeze-thaw injury. In Experiment I, 14 ejaculates from 10 Holstein bulls were held at 32°C for either 0, 20, 40, 60, 120 or 240 min followed by dilution in egg yolk-citrate. All treatments were maintained at 32°C for 240 min post-collection, at which time semen was cooled to 5°C, glycerolated, and then frozen in .5-ml French Straws using N₂ vapor. Experiment II, using 18 ejaculates from 10 bulls, was conducted identically to Experiment I, except semen was cooled to 5°C immediately after each dilution. Semen was thawed at 5°C and incubated at 37°C. Direct counts of intact acrosomes and estimates of percent motility were recorded at 0, 2, 4, 8 and 10 hrs of incubation. In Experiment I, there was a highly significant interaction (P < .01) between holding time x ejaculates to seminal plasma with regard to acrosomal retention. Optimum exposure time ranged from 20 to 120 min and 240-min exposure was deleterious for all ejaculates (P < .05). Variation in motility was not significant among treatments. In Experiment II, holding time x ejaculates interaction was again the most significant factor (P < .01). However, 20-min exposure to seminal plasma resulted in optimum acrosomal retention post-thaw for 15 ejaculates, while 40-min exposure was optimum for 2 ejaculates and 1 ejaculate did not respond favorably to any exposure time. While degree of response to seminal plasma exposure time varied among ejaculates, 20-min exposure was not deleterious to any ejaculate. Post-thaw motility was significantly (P < .01) reduced by 240-min exposure to seminal plasma.
    URI
    http://hdl.handle.net/10919/64685
    Collections
    • Masters Theses [20939]

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us
     

     

    VTechWorks

    AboutPoliciesHelp

    Browse

    All of VTechWorksCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Log inRegister

    Statistics

    View Usage Statistics

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us