Show simple item record

dc.contributor.authorMccoski, Sarah Reginaen_US
dc.date.accessioned2016-07-03T06:00:19Z
dc.date.available2016-07-03T06:00:19Z
dc.date.issued2015-01-09en_US
dc.identifier.othervt_gsexam:4183en_US
dc.identifier.urihttp://hdl.handle.net/10919/71709
dc.description.abstractEmbryonic wastage is rampant in cattle during early stages of pregnancy, particularly the first few weeks of gestation, a time recognized for significant remodeling of the embryo. Of particular interest to this laboratory are the first two lineage specification events, trophectoderm (TE) and primitive endoderm (PrE) specification, occurring between days 6 and 8 of gestation. The TE is responsible for uterine attachment and production of interferon-tau (IFNT), the factor of maternal recognition of pregnancy in ruminants. The PrE forms the yolk sac, which provides nutrients to the developing embryo. It is probable that developmental miscues during these differentiation events are responsible for the high rate of pregnancy loss, however, information on these early lineage processes is lacking in ruminants. The objective of the first study was to improve the current methods for detecting IFNT in biological samples. A novel interferon stimulatory response element (ISRE)-reporter assay was created, and provides adequate quantification to measure IFNT. Additionally, it has a shorter completion time than previous bioassays, and does not require the use of a live virus. The second study describes the development of a PrE cell line derived from bovine embryos. The PrE outgrowths can be produced at high rate, and can be maintained in a continuous culture system for about 6 weeks. As a true bovine PrE cell line does not currently exist, these lines hold great potential for the study of early development. Collectively, these studies improve knowledge of bovine embryogenesis, and provide insights that may be used to limit the pregnancy failures occurring in this species.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.rightsThis Item is protected by copyright and/or related rights. Some uses of this Item may be deemed fair and permitted by law even without permission from the rights holder(s), or the rights holder(s) may have licensed the work for use under certain conditions. For other uses you need to obtain permission from the rights holder(s).en_US
dc.subjectInterferonen_US
dc.subjectPrimitive endodermen_US
dc.titleDevelopment of an Interferon Bioassay and Primitive Endoderm Cell Lines to Study Lineage Specification During Early Bovine Embryogenesisen_US
dc.typeThesisen_US
dc.contributor.departmentAnimal and Poultry Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineAnimal and Poultry Sciencesen_US
dc.contributor.committeechairEaly, Alan Daleen_US
dc.contributor.committeememberGilbert, Elizabeth Ruthen_US
dc.contributor.committeememberRhoads, Michelleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record