Virginia Tech
    • Log in
    View Item 
    •   VTechWorks Home
    • VTechWorks Archives
    • VTechWorks Administration
    • All Faculty Deposits
    • View Item
    •   VTechWorks Home
    • VTechWorks Archives
    • VTechWorks Administration
    • All Faculty Deposits
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Ligand-receptor binding kinetics in surface plasmon resonance cells: A Monte Carlo analysis

    Thumbnail
    View/Open
    Submitted Version (3.028Mb)
    Downloads: 165
    Date
    2016-09-14
    Author
    Carroll, J.
    Raum, M.
    Forsten-Williams, Kimberly
    Täuber, Uwe C.
    Metadata
    Show full item record
    Abstract
    Surface plasmon resonance (SPR) chips are widely used to measure association and dissociation rates for the binding kinetics between two species of chemicals, e.g., cell receptors and ligands. It is commonly assumed that ligands are spatially well mixed in the SPR region, and hence a mean-field rate equation description is appropriate. This approximation however ignores the spatial fluctuations as well as temporal correlations induced by multiple local rebinding events, which become prominent for slow diffusion rates and high binding affinities. We report detailed Monte Carlo simulations of ligand binding kinetics in an SPR cell subject to laminar flow. We extract the binding and dissociation rates by means of the techniques frequently employed in experimental analysis that are motivated by the mean-field approximation. We find major discrepancies in a wide parameter regime between the thus extracted rates and the known input simulation values. These results underscore the crucial quantitative importance of spatio-temporal correlations in binary reaction kinetics in SPR cell geometries, and demonstrate the failure of a mean-field analysis of SPR cells in the regime of high Damk\"ohler number Da > 0.1, where the spatio-temporal correlations due to diffusive transport and ligand-receptor rebinding events dominate the dynamics of SPR systems.
    URI
    http://hdl.handle.net/10919/73062
    Collections
    • All Faculty Deposits [3559]
    • Scholarly Works, Department of Physics [845]

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us
     

     

    VTechWorks

    AboutPoliciesHelp

    Browse

    All of VTechWorksCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Log inRegister

    Statistics

    View Usage Statistics

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us