Virginia Tech
    • Log in
    View Item 
    •   VTechWorks Home
    • VTechWorks Archives
    • VTechWorks Administration
    • All Faculty Deposits
    • View Item
    •   VTechWorks Home
    • VTechWorks Archives
    • VTechWorks Administration
    • All Faculty Deposits
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Chemical Proteomic Platform To Identify Citrullinated Proteins

    Thumbnail
    View/Open
    Publisher's Version (2.681Mb)
    Downloads: 130
    Date
    2015-11-20
    Author
    Lewallen, Daniel M.
    Bicker, Kevin L.
    Subramanian, Venkataraman
    Clancy, Kathleen W.
    Slade, Daniel J.
    Martell, Julianne
    Dreyton, Christina J.
    Sokolove, Jeremy
    Weerapana, Eranthie
    Thompson, Paul R.
    Metadata
    Show full item record
    Abstract
    Anti-citrullinated protein antibodies (ACPAs) are a hallmark of rheumatoid arthritis (RA) and are routinely used for disease diagnosis. Protein citrullination is also increased in cancer and other autoimmune disorders, suggesting that citrullinated proteins may serve as biomarkers for diseases beyond RA. To identify these citrullinated proteins, we developed biotin-conjugated phenylglyoxal (biotin-PG). Using this probe and our platform technology, we identified >50 intracellular citrullinated proteins. More than 20 of these are involved in RNA splicing, suggesting, for the first time, that citrullination modulates RNA biology. Overall, this chemical proteomic platform will play a key role in furthering ourunderstanding of protein citrullination in rheumatoid arthritis and potentially a wider spectrum of inflammatory diseases.
    URI
    http://hdl.handle.net/10919/73406
    Collections
    • All Faculty Deposits [2306]
    • Scholarly Works, Department of Biochemistry [196]
    • Scholarly Works, Virginia Tech Center for Drug Discovery [35]

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us
     

     

    VTechWorks

    AboutPoliciesHelp

    Browse

    All of VTechWorksCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Log inRegister

    Statistics

    View Usage Statistics

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us