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dc.contributor.authorMiller, CBen_US
dc.contributor.authorPierlé, SAen_US
dc.contributor.authorBrayton, KAen_US
dc.contributor.authorOchoa, JNen_US
dc.contributor.authorShah, DHen_US
dc.contributor.authorLahmers, KKen_US
dc.coverage.spatialSwitzerlanden_US
dc.date.accessioned2016-11-16T01:45:06Z
dc.date.available2016-11-16T01:45:06Z
dc.date.issued2014en_US
dc.identifier.urihttp://hdl.handle.net/10919/73464
dc.description.abstractVaccination with Salmonella enterica serovar Typhimurium lacking DNA adenine methyltransferase confers cross-protective immunity against multiple Salmonella serotypes. The mechanistic basis is thought to be associated with the de-repression of genes that are tightly regulated when transiting from one microenvironment to another. This de-repression provides a potential means for the production of a more highly expressed and stable antigenic repertoire capable of inducing cross-protective immune responses. To identify genes encoding proteins that may contribute to cross-protective immunity, we used a Salmonella Typhimurium DNA adenine methyltransferase mutant strain (UK-1 dam mutant) derived from the parental UK-1 strain, and assessed the transcriptional profile of the UK-1 dam mutant and UK-1 strain grown under conditions that simulate the intestinal or endosomal microenvironments encountered during the infective process. As expected, the transcriptional profile of the UK-1 dam mutant identified a set of genes more transcriptionally active when compared directly to UK-1, and stably transcribed in biologically relevant culture conditions. Further, 22% of these genes were more highly transcribed in comparison to two other clinically-relevant Salmonella serovars. The strategy employed here helps to identify potentially conserved proteins produced by the UK-1 dam mutant that stimulate and/or modulate the development of cross-protective immune responses toward multiple Salmonella serotypes.en_US
dc.format.extent417 - 436 page(s)en_US
dc.languageENGen_US
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/25364573en_US
dc.subjectDNA adenine methyltransferaseen_US
dc.subjectSalmonella Typhimuriumen_US
dc.subjectbacteriophageen_US
dc.subjectfimbriaeen_US
dc.subjectimmunityen_US
dc.subjecttranscriptomeen_US
dc.titleTranscriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments.en_US
dc.typeArticle - Refereed
dc.description.notesPublished (Publication status)en_US
dc.title.serialPathogensen_US
dc.identifier.doihttps://doi.org/10.3390/pathogens3020417
dc.identifier.volume3en_US
dc.identifier.issue2en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences
pubs.organisational-group/Virginia Tech/Veterinary Medicine
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiology


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