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dc.contributor.authorWilliams, Sarah P.en_US
dc.contributor.authorRangarajan, Padmaen_US
dc.contributor.authorDonahue, Janet L.en_US
dc.contributor.authorHess, Jenna S.en_US
dc.contributor.authorGillaspy, Glenda E.en_US
dc.date.accessioned2017-01-04T22:09:54Z
dc.date.available2017-01-04T22:09:54Z
dc.date.issued2014-07-10en_US
dc.identifier.issn1664-462Xen_US
dc.identifier.urihttp://hdl.handle.net/10919/73944
dc.description.abstractThe Sucrose non-Fermenting Related Kinase 1( SnRK1) proteins have been linked to regulation of energy and stress signaling in eukaryotes. In plants, there is a small SnRK1 gene family. While the SnRK1.1 gene has been well studied, the role other SnRK1 isoforms play in energy or stress signaling is less well understood. We used promoter:GUS analysis and found SnRK1.1is broadly expressed, while SnRK1.2 is spatially restricted. SnRK1.2 is expressed most abundantly in hydathodes, at the base of leaf primordia, and in vascular tissues within both shoots and roots. We examined the impact that sugars have on SnRK1 gene expression and found that trehalose induces SnRK1.2 expression. Given that the SnRK1.1 and SnRK1.2 proteins are very similar at the amino acid level, we sought to address whether SnRK1.2 is capable of re-programming growth and development as has been seen previously with SnRK1.1 over expression. While gain-of-function transgenic plants over expressing two different isoforms of SnRK1.1 flower late as seen previously in other SnRK1.1 over expressors, SnRK1.2 over expressors flower early. In addition, SnRK1.2 over expressors have increased leaf size and rosette diameter during. Early development, which is the opposite of SnRK1.1 over expressors. We also investigated whether SnRK1.2 was localized to similar subcellular compartments as SnRK1.1, and found that both accumulate in the nucleus and cytoplasm in transient expression assays. In addition, we found SnRK1.1 accumulates in small puncta that appear after a mechanical wounding stress. Together, these data suggest key differences in regulation of the SnRK1.1 and SnRK1.2 genes in plants, and highlights differences over expression of each gene has on the development of Arabidopsis.en
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000339506700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 3.0 Unported (CC BY 3.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/*
dc.subjectSnRK1en_US
dc.subjecttrehaloseen_US
dc.subjectsugar-sensingen_US
dc.subjectenergyen_US
dc.subjectgene expressionen_US
dc.titleRegulation of Sucrose non-Fermenting Related Kinase 1 genes in Arabidopsis thalianaen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.rights.holderThe Author(s)en_US
dc.title.serialFRONTIERS IN PLANT SCIENCEen_US
dc.identifier.doihttps://doi.org/10.3389/fpls.2014.00324
dc.identifier.volume5en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistry
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/All T&R Faculty


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Creative Commons Attribution 3.0 Unported (CC BY 3.0)
License: Creative Commons Attribution 3.0 Unported (CC BY 3.0)