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dc.contributor.authorRigby, J. R.en_US
dc.contributor.authorPoelzing, Stevenen_US
dc.coverage.spatialUnited Statesen_US
dc.description.abstractVoltage clamping is an important tool for measuring individual currents from an electrically active cell. However, it is difficult to isolate individual currents without pharmacological or voltage inhibition. Herein, we present a technique that involves inserting a noise function into a standard voltage step protocol, which allows one to characterize the unique frequency response of an ion channel at different step potentials. Specifically, we compute the fast Fourier transform for a family of current traces at different step potentials for the inward rectifying potassium channel, K(ir)2.1, and the channel encoding the cardiac fast sodium current, Na(v)1.5. Each individual frequency magnitude, as a function of voltage step, is correlated to the peak current produced by each channel. The correlation coefficient vs. frequency relationship reveals that these two channels are associated with some unique frequencies with high absolute correlation. The individual IV relationship can then be recreated using only the unique frequencies with magnitudes of high absolute correlation. Thus, this study demonstrates that ion channels may exhibit unique frequency responses.en_US
dc.format.extent946 - 954 page(s)en_US
dc.rightsIn Copyrighten
dc.subjectDielectric Spectroscopyen_US
dc.subjectHEK293 Cellsen_US
dc.subjectIon Transporten_US
dc.subjectMembrane Potentialsen_US
dc.subjectNAV1.5 Voltage-Gated Sodium Channelen_US
dc.subjectPotassium Channels, Inwardly Rectifyingen_US
dc.subjectSodium Channelsen_US
dc.titleA novel frequency analysis method for assessing K(ir)2.1 and Na (v)1.5 currents.en_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.contributor.departmentBiomedical Engineering and Mechanicsen_US
dc.contributor.departmentFralin Biomedical Research Institute at VTCen_US
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen_US
dc.title.serialAnn Biomed Engen_US
dc.type.otherResearch Support, N.I.H., Extramuralen_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes
pubs.organisational-group/Virginia Tech/University Research Institutes/Virginia Tech Carilion Research Institute

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