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dc.contributorVirginia Techen
dc.contributor.authorGuo, Weihuaen
dc.contributor.authorSheng, Jiayuanen
dc.contributor.authorFeng, Xueyangen
dc.description.abstractWith the breakthroughs in biomolecular engineering and synthetic biology, many valuable biologically active compound and commodity chemicals have been successfully manufactured using cell-based approaches in the past decade. However, because of the high complexity of cell metabolism, the identification and optimization of rate-limiting metabolic pathways for improving the product yield is often difficult, which represents a significant and unavoidable barrier of traditional in vivo metabolic engineering. Recently, some in vitro engineering approaches were proposed as alternative strategies to solve this problem. In brief, by reconstituting a biosynthetic pathway in a cell-free environment with the supplement of cofactors and substrates, the performance of each biosynthetic pathway could be evaluated and optimized systematically. Several value-added products, including chemicals, nutraceuticals, and drug precursors, have been biosynthesized as proof-of-concept demonstrations of in vitro metabolic engineering. This mini-review summarizes the recent progresses on the emerging topic of in vitro metabolic engineering and comments on the potential application of cell-free technology to speed up the “design-build-test” cycles of biomanufacturing.en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.subjectMetabolic pathwaysen
dc.subjectDesign-build-test cycleen
dc.titleMini-review: In vitro Metabolic Engineering for Biomanufacturing of High-value Productsen
dc.typeArticle - Refereeden
dc.contributor.departmentBiological Systems Engineeringen
dc.title.serialComputational and Structural Biotechnology Journalen

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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International