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dc.contributor.authorArtemov, Gleb N.en_US
dc.contributor.authorPeery, Ashley N.en_US
dc.contributor.authorJiang, Xiaofangen_US
dc.contributor.authorTu, Zhijianen_US
dc.contributor.authorStegniy, Vladimir N.en_US
dc.contributor.authorSharakhova, Maria V.en_US
dc.contributor.authorSharakhov, Igor V.en_US
dc.date.accessioned2018-01-02T23:12:42Z
dc.date.available2018-01-02T23:12:42Z
dc.date.issued2017-01-01en_US
dc.identifier.issn2160-1836en_US
dc.identifier.urihttp://hdl.handle.net/10919/81473
dc.description.abstractThe genome of the Neotropical malaria vector Anopheles albimanus was sequenced as part of the 16 Anopheles Genomes Project published in 2015. The draft assembly of this species consisted of 204 scaffolds with an N50 scaffold size of 18.1 Mb and a total assembly size of 170.5 Mb. It was among the smallest genomes with the longest scaffolds in the 16 Anopheles species cluster, making An. albimanus the logical choice for anchoring the genome assembly to chromosomes. In this study, we developed a high-resolution cytogenetic photomap with completely straightened polytene chromosomes from the salivary glands of the mosquito larvae. Based on this photomap, we constructed a chromosome-based genome assembly using fluorescent in situ hybridization of PCR-amplified DNA probes. Our physical mapping, assisted by an ortholog-based bioinformatics approach, identified and corrected nine misassemblies in five large genomic scaffolds.Misassemblies mostly occurred in junctions between contigs. Our comparative analysis of scaffolds with the An. gambiae genome detected multiple genetic exchanges between pericentromeric regions of chromosomal arms caused by partial-arm translocations. The finalmap consists of 40 ordered genomic scaffolds and corrected fragments ofmisassembled scaffolds. The An. albimanus physical map comprises 98.2% of the total genome assembly and represents the most complete genome map among mosquito species. This study demonstrates that physical mapping is a powerful tool for correcting errors in draft genome assemblies and for creating chromosome-anchored reference genomes.en
dc.format.extent155 - 164 (10) page(s)en_US
dc.languageEnglishen_US
dc.publisherGenetics Society Americaen_US
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000392200800014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en_US
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectGenetics & Heredityen_US
dc.subjectmosquito genomeen_US
dc.subjectfluorescence in situ hybridizationen_US
dc.subjectphysical mappingen_US
dc.subjectpolytene chromosomesen_US
dc.subjectAnopheles albimanusen_US
dc.subjectIN-SITU HYBRIDIZATIONen_US
dc.subjectMALARIA VECTORen_US
dc.subjectCHROMOSOMAL INVERSIONSen_US
dc.subjectPOLYTENE CHROMOSOMESen_US
dc.subjectCYTOGENETIC MAPen_US
dc.subjectAEDES-AEGYPTIen_US
dc.subjectLINKAGE MAPen_US
dc.subjectMOSQUITOen_US
dc.subjectFUNESTUSen_US
dc.subjectGAMBIAEen_US
dc.titleThe Physical Genome Mapping of Anopheles albimanus Corrected Scaffold Misassemblies and Identified Interarm Rearrangements in Genus Anophelesen_US
dc.typeArticle - Refereed
dc.description.versionPublished (Publication status)en_US
dc.rights.holderThe Author(s)en_US
dc.title.serialG3-GENES GENOMES GENETICSen_US
dc.identifier.doihttps://doi.org/10.1534/g3.116.034959
dc.identifier.volume7en_US
dc.identifier.issue1en_US
dc.identifier.orcidSharakhov, IV [0000-0003-0752-3747]en_US
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistry
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomology
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Faculty


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Creative Commons Attribution 4.0 International (CC BY 4.0)
License: Creative Commons Attribution 4.0 International (CC BY 4.0)