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dc.contributor.authorLiu, Qiuxu
dc.contributor.authorQi, Xiao
dc.contributor.authorYan, Haidong
dc.contributor.authorHuang, Linkai
dc.contributor.authorNie, Gang
dc.contributor.authorZhang, Xinquan
dc.date.accessioned2018-01-25T17:52:02Z
dc.date.available2018-01-25T17:52:02Z
dc.date.issued2018-01-16
dc.identifier.citationLiu, Q.; Qi, X.; Yan, H.; Huang, L.; Nie, G.; Zhang, X. Reference Gene Selection for Quantitative Real-Time Reverse-Transcriptase PCR in Annual Ryegrass (Lolium multiflorum) Subjected to Various Abiotic Stresses. Molecules 2018, 23, 172.
dc.identifier.urihttp://hdl.handle.net/10919/81929
dc.description.abstractTo select the most stable reference genes in annual ryegrass (Lolium multiflorum), we studied annual ryegrass leaf tissues exposed to various abiotic stresses by qRT-PCR and selected 11 candidate reference genes, i.e., 18S rRNA, E2, GAPDH, eIF4A, HIS3, SAMDC, TBP-1, Unigene71, Unigene77, Unigene755, and Unigene14912. We then used GeNorm, NormFinder, and BestKeeper to analyze the expression stability of these 11 genes, and used RefFinder to comprehensively rank genes according to stability. Under different stress conditions, the most suitable reference genes for studies of leaf tissues of annual ryegrass were different. The expression of the eIF4A gene was the most stable under drought stress. Under saline-alkali stress, Unigene14912 has the highest expression stability. Under acidic aluminum stress, SAMDC expression stability was highest. Under heavy metal stress, Unigene71 expression had the highest stability. According to the software analyses, Unigene14912, HIS3, and eIF4A were the most suitable for analyses of abiotic stress in tissues of annual ryegrass. GAPDH was the least suitable reference gene. In conclusion, selecting appropriate reference genes under abiotic stress not only improves the accuracy of annual ryegrass gene expression analyses, but also provides a theoretical reference for the development of reference genes in plants of the genus Lolium.
dc.format.mimetypeapplication/pdf
dc.languageen_USen_US
dc.publisherMDPI
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleReference Gene Selection for Quantitative Real-Time Reverse-Transcriptase PCR in Annual Ryegrass (Lolium multiflorum) Subjected to Various Abiotic Stressesen_US
dc.typeArticle - Refereeden_US
dc.date.updated2018-01-24T21:04:01Z
dc.title.serialMolecules
dc.identifier.doihttps://doi.org/10.3390/molecules23010172
dc.type.dcmitypeText


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International