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dc.contributor.authorCahuascanco, Berlyen
dc.contributor.authorBahamonde, Javieraen
dc.contributor.authorHuaman, Olgeren
dc.contributor.authorJervis, Miguelen
dc.contributor.authorCortez, Jahairaen
dc.contributor.authorPalomino, Jaimeen
dc.contributor.authorEscobar, Alejandroen
dc.contributor.authorRetamal, Patricioen
dc.contributor.authorTorres, Cristian G.en
dc.contributor.authorPeralta, Oscar A.en
dc.date.accessioned2019-04-15T11:54:49Zen
dc.date.available2019-04-15T11:54:49Zen
dc.date.issued2019-04-11en
dc.identifier.citationVeterinary Research. 2019 Apr 11;50(1):25en
dc.identifier.urihttp://hdl.handle.net/10919/88960en
dc.description.abstractStaphylococcus aureus is the most commonly isolated pathogen from clinical bovine mastitis samples and a difficult pathogen to combat. Mesenchymal stem cells (MSC) are multipotent progenitor cells equipped with a variety of factors that inhibit bacterial growth. The aim of the present study was to evaluate the in vitro antibacterial potential against S. aureus of conditioned medium (CM) from MSC derived from fetal bovine bone marrow (BM-MSC) and adipose tissue (AT-MSC). BM-MSC, AT-MSC and fetal fibroblasts (FB) cultures were activated by infection with S. aureus. Bacterial growth was evaluated in presence of CM, concentrated CM (CCM), activated CM (ACM) and concentrated ACM (CACM) from BM-MSC, AT-MSC and FB. Gene expression of β-defensin 4A (bBD-4A), NK-lysine 1 (NK1), cathelicidin 2 (CATHL2), hepcidin (HEP) and indoleamine 2,3 dioxygenase (IDO) and protein expression of bBD-4A were determined in activated and non-activated cells. The majority of BM-MSC and AT-MSC expressed CD73, Oct4 and Nanog, and were negative for CD34. Growth of S. aureus decreased when it was exposed to CM from BM-MSC, AT-MSC and FB. Moreover, growth of S. aureus in CCM, ACM and CACM was lower compared to controls of CM from BM-MSC and AT-MSC. Activated AT-MSC increased mRNA levels of bBD4A and NK1, and protein levels of bBD4A in CM. Thus, CM from fetal bovine BM-MSC and AT-MSC has the capacity to reduce in average ~30% of S. aureus relative growth under in vitro conditions. The in vitro antibacterial effect of fetal bovine MSC may be mediated by bBD4A and NK1 activity.en
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleBovine fetal mesenchymal stem cells exert antiproliferative effect against mastitis causing pathogen Staphylococcus aureusen
dc.typeArticle - Refereeden
dc.date.updated2019-04-14T03:34:04Zen
dc.description.versionPublished versionen
dc.rights.holderThe Author(s)en
dc.title.serialVeterinary Researchen
dc.identifier.doihttps://doi.org/10.1186/s13567-019-0643-1en
dc.type.dcmitypeTexten


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License: Creative Commons Attribution 4.0 International