Changes in RNA Splicing in Developing Soybean (Glycine max) Embryos

dc.contributor.authorAghamirzaie, Delasaen
dc.contributor.authorNabiyouni, Mahdien
dc.contributor.authorFang, Yihuien
dc.contributor.authorKlumas, Curtisen
dc.contributor.authorHeath, Lenwood S.en
dc.contributor.authorGrene, Ruthen
dc.contributor.authorCollakova, Evaen
dc.contributor.departmentElectrical and Computer Engineeringen
dc.contributor.departmentComputer Scienceen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessed2014-01-06en
dc.date.accessioned2014-01-15T14:16:34Zen
dc.date.available2014-01-15T14:16:34Zen
dc.date.issued2013-11-21en
dc.description.abstractDeveloping soybean seeds accumulate oils, proteins, and carbohydrates that are used as oxidizable substrates providing metabolic precursors and energy during seed germination. The accumulation of these storage compounds in developing seeds is highly regulated at multiple levels, including at transcriptional and post-transcriptional regulation. RNA sequencing was used to provide comprehensive information about transcriptional and post-transcriptional events that take place in developing soybean embryos. Bioinformatics analyses lead to the identification of different classes of alternatively spliced isoforms and corresponding changes in their levels on a global scale during soybean embryo development. Alternative splicing was associated with transcripts involved in various metabolic and developmental processes, including central carbon and nitrogen metabolism, induction of maturation and dormancy, and splicing itself. Detailed examination of selected RNA isoforms revealed alterations in individual domains that could result in changes in subcellular localization of the resulting proteins, protein-protein and enzyme-substrate interactions, and regulation of protein activities. Different isoforms may play an important role in regulating developmental and metabolic processes occurring at different stages in developing oilseed embryos.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAghamirzaie, Delasa; Nabiyouni, Mahdi; Fang, Yihui; Klumas, Curtis; Heath, Lenwood S.; Grene, Ruth; Collakova, Eva. 2013. "Changes in RNA Splicing in Developing Soybean (Glycine max) Embryos." Biology 2013, 2(4), 1311-1337; doi:10.3390/biology2041311.en
dc.identifier.doihttps://doi.org/10.3390/biology2041311en
dc.identifier.issn2079-7737en
dc.identifier.urihttp://hdl.handle.net/10919/24834en
dc.identifier.urlhttp://www.mdpi.com/2079-7737/2/4/1311en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAbscisic aciden
dc.subjectAlternative splicingen
dc.subjectAuxinen
dc.subjectCentral carbon and nitrogen metabolismen
dc.subjectDesiccation toleranceen
dc.subjectDormancy inductionen
dc.subjectPost-transcriptional regulationen
dc.subjectSeed and embryo developmenten
dc.subjectSoybeanen
dc.titleChanges in RNA Splicing in Developing Soybean (Glycine max) Embryosen
dc.title.serialBiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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