Resolving bacteria with the coulter counter

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dc.contributor.authorJacobson, Ronald Leslieen
dc.contributor.committeechairBungay, Henry R. IIIen
dc.contributor.committeememberParsons, William A.en
dc.contributor.committeememberBeckett, Mauriceen
dc.contributor.departmentWater Technologyen
dc.date.accessioned2014-03-14T21:49:17Zen
dc.date.adate2012-11-09en
dc.date.available2014-03-14T21:49:17Zen
dc.date.issued1964-08-05en
dc.date.rdate2012-11-09en
dc.date.sdate2012-11-09en
dc.description.abstractPresent methods of counting and sizing of bacteria arc usually tedious and often have low statistical validity. The objective of this thesis was to investigate the feasibility of counting and sizing of bacteria with the Coulter Counter and to evaluate orifices which were prepared in this laboratory. Size distributions of Pasteurella multocida and Proteus vulgaris were obtained with the electronic particle counter. These distributions appeared to follow the normal distribution when relative per cent was plotted against diameter, The two modes of the distributions appeared far enough apart to warrant future attempts to separate quantitatively <i>P. vulgaris and P. multocida </i>in mixed suspensions. Size distributions also were obtained for <i>Staphylococcus epidermidis, Sarcina lutea,</i> and <i>Azotobacter</i>. Many variables appeared to be inherent in the electronic particle method of counting biological cells; among these are the biological cells, diluent, and electronic circuitry. Apparently the variability in the electronic circuitry caused the calibration of laboratory orifices to be impossible according to conventional methods.en
dc.description.degreeMaster of Scienceen
dc.format.extent84 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-11092012-040148en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11092012-040148/en
dc.identifier.urihttp://hdl.handle.net/10919/45562en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1964.J326.pdfen
dc.relation.isformatofOCLC# 20720644en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1964.J326en
dc.subject.lcshBacteriologyen
dc.subject.lcshWater -- Microbiologyen
dc.titleResolving bacteria with the coulter counteren
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineWater Technologyen
thesis.degree.grantorVirginia Polytechnic Instituteen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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