Airborne Campylobacter in a Poultry Processing Plant

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dc.contributor.authorJohnson, Anjeanette Christinaen
dc.contributor.committeechairEifert, Joseph D.en
dc.contributor.committeememberMcElroy, Audrey P.en
dc.contributor.committeememberWilliams, Robert C.en
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2014-03-14T20:36:32Zen
dc.date.adate2010-05-25en
dc.date.available2014-03-14T20:36:32Zen
dc.date.issued2010-05-29en
dc.date.rdate2010-05-25en
dc.date.sdate2010-05-12en
dc.description.abstractCampylobacter is a foodborne pathogen commonly found in live poultry and raw poultry products. Identifying areas of contamination or modes of transmission during commercial processing can lead to strategies to reduce the level of Campylobacter on finished products. Monitoring levels of airborne Campylobacter may be useful for identifying the presence or relative concentration of the pathogen in a processing plant environment. In this study, air sampling was used to detect and quantify Campylobacter in a commercial chicken processing plant by location within the plant and collection time during the day. Air was sampled from evisceration and post-chill areas in a poultry processing plant on four days and at 4 hour intervals onto Campy-Cefex agar plates or gelatin filters that were subsequently transferred to Campy-Cefex agar plates. Additionally, pre-evisceration and post-chill carcass rinses were analyzed quantitatively for Campylobacter. The mean level of airborne Campylobacter was 5 CFU/1000L of air sampled (10% samples positive) in comparison with 413 CFU/mL from carcass rinses (70% samples positive). Higher concentrations were found in carcass rinse samples from pre-evisceration. Airborne Campylobacter was detected from the evisceration area more frequently than from the post-chill carcass area of the plant (P < 0.05). This study shows that airborne Campylobacter can be quantified with a selective agar and with gelatin filter collection. Further research is needed to prove the utility of airborne detection of Campylobacter for estimating the relative contamination level of live poultry flocks and the processing plant environment and the potential for cross-contamination.en
dc.description.degreeMaster of Science in Life Sciencesen
dc.identifier.otheretd-05122010-234510en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05122010-234510/en
dc.identifier.urihttp://hdl.handle.net/10919/32668en
dc.publisherVirginia Techen
dc.relation.haspartJohnson_AC_T_2010_.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectCampylobacteren
dc.subjectair samplingen
dc.subjectcarcass rinseen
dc.subjectpoultryen
dc.subjectgelatin filteren
dc.subjectCampy-Cefexen
dc.titleAirborne Campylobacter in a Poultry Processing Planten
dc.typeThesisen
thesis.degree.disciplineFood Science and Technologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Science in Life Sciencesen

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