Epigallocatechin Gallate in the Regulation of Insulin Secretion

dc.contributor.authorYuskavage, Julia Kathrynen
dc.contributor.committeechairLiu, Dongminen
dc.contributor.committeememberGwazdauskas, Francis C.en
dc.contributor.committeememberBarbeau, William E.en
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2014-03-14T20:36:48Zen
dc.date.adate2008-06-06en
dc.date.available2014-03-14T20:36:48Zen
dc.date.issued2008-04-30en
dc.date.rdate2010-12-22en
dc.date.sdate2008-05-14en
dc.description.abstractIn both Type 1 diabetes (T1D) and Type 2 diabetes (T2D), inadequate beta-cell mass and beta-cell dysfunction lead to impaired insulin secretion, and ultimately worsen glycemic control. Green tea has drawn wide attention due to its possible health-promoting properties, including enhancement of beta-cell function. We assessed the acute and relative long-term effects of epigallocatechin gallate (EGCG) on insulin secretion and synthesis from clonal beta-cells (INS1E cells), rat islets, and human islets, using 0.1, 1, or 5 µM. We determined if EGCG decreased blood glucose in healthy rats acutely, using 50 or 150 mg/kg body weight (BW), and after 12 days of supplementation in drinking water, using 0.1% and 0.5%. In the in vitro studies, EGCG significantly potentiated glucose-stimulated insulin secretion (GSIS) in rat islets (at 0.1, 1, and 5 µM) and human islets (at 1 µM), and elevated insulin content within INS1E cells (at 0.1, 1, and 5 µm) and human islets (at 1 µM), (P<0.05). Nutritional supplementation of EGCG (0.5% in drinking water) for 12 days in healthy rats significantly increased insulin synthesis, compared to that of controls, from 0.2 ± 0.02 to 1.4 ± 0.2 ng/mg protein, without alteration of insulin secretion in isolated islets (P<0.05). These findings demonstrate that EGCG may play a role in the regulation of pancreatic beta-cell function, thereby contributing to an anti-diabetic effect of this agent.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05142008-162857en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05142008-162857/en
dc.identifier.urihttp://hdl.handle.net/10919/32761en
dc.publisherVirginia Techen
dc.relation.haspartJuliaYuskavageRevisedETD.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectinsulinen
dc.subjectcatechinen
dc.subjectdiabetesen
dc.subjectgreen teaen
dc.subjectEGCGen
dc.subjectisletsen
dc.subjectpancreatic β-cellen
dc.titleEpigallocatechin Gallate in the Regulation of Insulin Secretionen
dc.typeThesisen
thesis.degree.disciplineHuman Nutrition, Foods, and Exerciseen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen
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