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Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome

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dc.contributor.authorNaumenko, Anastasia N.en
dc.contributor.authorTimoshevskiy, Vladimir A.en
dc.contributor.authorKinney, Nicholas A.en
dc.contributor.authorKokhanenko, Alina A.en
dc.contributor.authordeBruyn, Becky S.en
dc.contributor.authorLovin, Diane D.en
dc.contributor.authorStegniy, Vladimir N.en
dc.contributor.authorSeverson, David W.en
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.authorSharakhova, Maria V.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2017-01-05T22:13:15Zen
dc.date.available2017-01-05T22:13:15Zen
dc.date.issued2015-03-13en
dc.description.abstractThe genome assembly of southern house mosquito Cx. quinquefasciatus is represented by a high number of supercontigs with no order or orientation on the chromosomes. Although cytogenetic maps for the polytene chromosomes of this mosquito have been developed, their utilization for the genome mapping remains difficult because of the low number of high quality spreads in chromosome preparations. Therefore, a simple and robust mitotic-chromosome-based approach for the genome mapping of Cx. quinquefasciatus still needs to be developed. In this study, we performed physical mapping of 37 genomic supercontigs using fluorescent in situ hybridization on mitotic chromosomes from imaginal discs of 4th instar larvae. The genetic linkage map nomenclature was adopted for the chromosome numbering based on the direct positioning of 58 markers that were previously genetically mapped. The smallest, largest, and intermediate chromosomes were numbered as 1, 2, and 3, respectively. For idiogram development, we analyzed and described in detail the morphology and proportions of the mitotic chromosomes. Chromosomes were subdivided into 19 divisions and 72 bands of four different intensities. These idiograms were used for mapping the genomic supercontigs/genetic markers. We also determined the presence of length polymorphism in the q arm of sex-determining chromosome 1 in Cx. quinquefasciatus related to the size of ribosomal locus. Our physical mapping and previous genetic linkage mapping resulted in the chromosomal assignment of 13% of the total genome assembly to the chromosome bands.We provided the first detailed description, nomenclature, and idiograms for the mitotic chromosomes of Cx. quinquefasciatus. Further application of the approach developed in this study will help to improve the quality of the southern house mosquito genome.en
dc.description.versionPublished versionen
dc.format.extent? - ? (13) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0115737en
dc.identifier.issn1932-6203en
dc.identifier.issue3en
dc.identifier.urihttp://hdl.handle.net/10919/73959en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherPLOSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000351277500002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectquantitative trait locien
dc.subjectmosquito aedes-aegyptien
dc.subjectmalaria parasite susceptibilityen
dc.subjecttubule polytene chromosomesen
dc.subjectanopheles-gambiaeen
dc.subjectpipiens complexen
dc.subjectlinkage mapen
dc.subjectdipteraen
dc.subjectculicidaeen
dc.subjectsequenceen
dc.titleMitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genomeen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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