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Effect of processing parameters on the detection of animal drug residues in milk

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dc.contributor.authorConner, Tonya Micheleen
dc.contributor.committeechairBishop, J. Russellen
dc.contributor.committeememberDuncan, Susan E.en
dc.contributor.committeememberJones, Gerald M.en
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2014-03-14T21:32:05Zen
dc.date.adate2010-03-17en
dc.date.available2014-03-14T21:32:05Zen
dc.date.issued1992-08-05en
dc.date.rdate2010-03-17en
dc.date.sdate2010-03-17en
dc.description.abstractThe advent of new methods to detect animal drug residues has resulted in a need to independently validate them. The effects of processing milk on the performance of these methods was evaluated. Antibiotic-free milk samples were spiked with sulfamethazine, penicillin G, and chlortetracycline at levels of 10, 10 and 30 ppb, respectively. Spiked milk and negative control milk was heat-treated, homogenized or heat-treated and homogenized. The procedures evaluated for penicillin detection were Bacillus stearothermophilus disk assay, a HPLC described by Barker et al., Charm II microbial receptor assay, and CITE Probe and LacTek enzyme immunoassays. The procedures evaluated for sulfonamide detection were an HPLC method described by Long et al., Charm II microbial receptor assay, CITE Probe, LacTek and Signal enzyme immunoassays. The methods evaluated for tetracycline detection were a HPLC method described by Long et al., Charm II microbial receptor assay, and LacTek and CITE Probe enzyme immunoassays. The results indicate that the commercial tests and the disk assay were not adversely affected by processing treatments. Significant treatment differences were found when testing raw Charm II data by analysis of variance but these differences did not effect the overall results of the test. Results of the HPLC method were inconclusive for the three drugs tested.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 74 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-03172010-020648en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-03172010-020648/en
dc.identifier.urihttp://hdl.handle.net/10919/41720en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1992.C667.pdfen
dc.relation.isformatofOCLC# 27407942en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1992.C667en
dc.subject.lcshAntibiotic residuesen
dc.subject.lcshMilken
dc.titleEffect of processing parameters on the detection of animal drug residues in milken
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineFood Science and Technologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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