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Xenogeneic transplantation of immortalized bovine mammary epithelial cells

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dc.contributor.authorEllis, Steven E.en
dc.contributor.committeechairAkers, Robert Michaelen
dc.contributor.committeememberElgert, Klaus D.en
dc.contributor.committeememberGwazdauskas, Francis C.en
dc.contributor.committeememberVinson, William E.en
dc.contributor.departmentDairy Scienceen
dc.date.accessioned2014-03-14T21:51:15Zen
dc.date.adate2009-12-05en
dc.date.available2014-03-14T21:51:15Zen
dc.date.issued1994-12-05en
dc.date.rdate2009-12-05en
dc.date.sdate2009-12-05en
dc.description.abstractThe focus of this research was to investigate the use of an immortalized bovine mammary epithelial cell line as a starting material for xenogeneic transplantation into the mammary glands of immunocompetent recipients. PSG-5 cells (a clonal derivative of the MAC-T cell line engineered to express ovine IGF-I ) were transplanted into the cleared mammary fat pads of recipient mice. Following transplantation, spheroidal cell structures were observed in the cleared mammary fat pads of immunocompetent control mice and in mice exposed to PSG-5 cells during fetal development. Spheroidal cell structures were not observed in glands that had not received cell transplants. The success of this xenogeneic transplantation prompted the development of a MAC-T cell clone expressing a bacterial β-galactosidase (βMAC-T's) for use as a histological marker protein. Studies were then performed to determine the most appropriate age and location for cell transplantation into ovine recipients. Between three and 12 weeks of age, parenchymal volume in the mammary glands collected from e\ves in this study (n=4/age group: 3,6,9, and 12 weeks) increased nearly lO-fold (3.8cm³ to 34.5cm³ ). Total gland volume increased approximately 5-fold (67.3cm³ to 316.2cm³ ). Based on these determinations of parenchymal and glandular volumes, we determined that transplantation should begin with lambs at about three weeks of age. This data provides a starting point to begin trials using βMAC-T cells, which have been engineered to express a histological marker protein, for transplantation into intact ovine and murine mammary glands.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 75 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-12052009-020330en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-12052009-020330/en
dc.identifier.urihttp://hdl.handle.net/10919/46164en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1994.E555.pdfen
dc.relation.isformatofOCLC# 32180302en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1994.E555en
dc.subject.lcshCell transplantationen
dc.subject.lcshEpithelial cellsen
dc.titleXenogeneic transplantation of immortalized bovine mammary epithelial cellsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineDairy Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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