Histological evaluation of ovine mammary tissue xenografted into cyclosporine treated mice

dc.contributor.authorDaniel, Thomas Earlen
dc.contributor.committeechairAkers, Robert Michaelen
dc.contributor.committeememberBarnes, Michael A.en
dc.contributor.committeememberMcGilliard, Michael L.en
dc.contributor.committeememberSaacke, Richard G.en
dc.contributor.committeememberVinson, William E.en
dc.contributor.departmentDairy Scienceen
dc.date.accessioned2014-03-14T21:39:00Zen
dc.date.adate2010-06-22en
dc.date.available2014-03-14T21:39:00Zen
dc.date.issued1988-02-18en
dc.date.rdate2010-06-22en
dc.date.sdate2010-06-22en
dc.description.abstractCyclosporine (CsA), a fungal peptide with remarkable anti-lymphocytic properties, has been shown to be an effective immunosuppressive agent. In three separate experiments, mice receiving CsA at various doses (8 mg/kg/day in the first two experiments and 70-100 mg/kg/day in the third), were implanted with ovine mammary tissue expants at various sites (subcutaneous dorsum, ovarian fat-pad, and cleared mammary gland fat-pad). After different periods of time, (day 3, 7, 14, 21, and 35) implants were removed, fixed, stained, and embedded. Epithelial morphology of the implants was evaluated via a grading system that classified epithelium from 1 (poor) to 5 (excellent). Leukocyte type and number was determined in subepithelial stroma of implants in the third experiment. In the first two experiments, histological evaluation revealed that the transplanted tissue did not survive, regardless of site of implantation or developmental stage of donor tissue. In experiment three higher, dosages of CsA and different donor tissue were used, this resulted in significantly higher (P < .05) mean epithelial scores (indicative of healthier epithelium) for two CsA treated groups vs two control groups (3.75 and 2.33 vs 2.0 and 1.25, respectively). Within each treatment group (CsA and control), one group of mice received exogenous estrogen and progesterone (EjP) implants. CsA treated mice receiving E/P had significantly higher (P < .05) epithelial scores than those not receiving hormones (3.68 vs 2.83). The reverse was true for lymphocyte number (18.9 vs 40.5 cells per 117,600 um² of subepithelial stroma) which were significantly less in CsA + E/P treated mice. The overall correlation between lymphocyte number and epithelial score was -.55 (P < .0001). These results indicate that ovine mammary tissue xenografted into mice treated with high doses of CsA, are capable of surviving for extended periods of time (at least 35 days).en
dc.description.degreeMaster of Scienceen
dc.format.extentxi, 91 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06222010-020225en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06222010-020225/en
dc.identifier.urihttp://hdl.handle.net/10919/43398en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1988.D365.pdfen
dc.relation.isformatofOCLC# 17796904en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1988.D365en
dc.subject.lcshMammary glandsen
dc.subject.lcshSheep -- Milk yielden
dc.titleHistological evaluation of ovine mammary tissue xenografted into cyclosporine treated miceen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineDairy Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen
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