The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression

dc.contributor.authorLai, Yanen
dc.contributor.authorLu, Xueqing Maggieen
dc.contributor.authorDaron, Josquinen
dc.contributor.authorPan, Songqinen
dc.contributor.authorWang, Jianqiangen
dc.contributor.authorWang, Weien
dc.contributor.authorTsuchiya, Tokujien
dc.contributor.authorHolub, Ericen
dc.contributor.authorMcDowell, John M.en
dc.contributor.authorSlotkin, R. Keithen
dc.contributor.authorLe Roch, Karine G.en
dc.contributor.authorEulgem, Thomasen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2021-09-07T14:36:39Zen
dc.date.available2021-09-07T14:36:39Zen
dc.date.issued2020-09-01en
dc.date.updated2021-09-07T14:36:34Zen
dc.description.abstractPlant NLR-type receptors serve as sensitive triggers of host immunity. Their expression has to be well-balanced, due to their interference with various cellular processes and dose-dependency of their defense-inducing activity. A genetic “arms race” with fast-evolving pathogenic microbes requires plants to constantly innovate their NLR repertoires. We previously showed that insertion of the COPIA-R7 retrotransposon into RPP7 co-opted the epigenetic transposon silencing signal H3K9me2 to a new function promoting expression of this Arabidopsis thaliana NLR gene. Recruitment of the histone binding protein EDM2 to COPIA-R7-associated H3K9me2 is required for optimal expression of RPP7. By profiling of genome-wide effects of EDM2, we now uncovered additional examples illustrating effects of transposons on NLR gene expression, strongly suggesting that these mobile elements can play critical roles in the rapid evolution of plant NLR genes by providing the “raw material” for gene expression mechanisms. We further found EDM2 to have a global role in NLR expression control. Besides serving as a positive regulator of RPP7 and a small number of other NLR genes, EDM2 acts as a suppressor of a multitude of additional NLR genes. We speculate that the dual functionality of EDM2 in NLR expression control arose from the need to compensate for fitness penalties caused by high expression of some NLR genes by suppression of others. Moreover, we are providing new insights into functional relationships of EDM2 with its interaction partner, the RNA binding protein EDM3/AIPP1, and its target gene IBM1, encoding an H3K9-demethylase.en
dc.description.versionPublished versionen
dc.format.extent31 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN e1008993 (Article number)en
dc.identifier.doihttps://doi.org/10.1371/journal.pgen.1008993en
dc.identifier.eissn1553-7404en
dc.identifier.issn1553-7404en
dc.identifier.issue9en
dc.identifier.orcidMcDowell, John [0000-0002-9070-4874]en
dc.identifier.otherPGENETICS-D-19-01617 (PII)en
dc.identifier.pmid32925902en
dc.identifier.urihttp://hdl.handle.net/10919/104940en
dc.identifier.volume16en
dc.language.isoenen
dc.publisherPLoSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000573373800006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectGenetics & Heredityen
dc.subjectDOWNY MILDEW RESISTANCEen
dc.subjectNB-LRR GENESen
dc.subjectDNA METHYLATIONen
dc.subjectDISEASE RESISTANCEen
dc.subjectPERONOSPORA-PARASITICAen
dc.subjectSALICYLIC-ACIDen
dc.subjectTRANSPOSABLE ELEMENTSen
dc.subjectLTR-RETROTRANSPOSONSen
dc.subjectENCODING GENESen
dc.subjectJMJC DOMAINen
dc.subjectDevelopmental Biologyen
dc.subject0604 Geneticsen
dc.subject.meshPlants, Genetically Modifieden
dc.subject.meshArabidopsisen
dc.subject.meshRNA-Binding Proteinsen
dc.subject.meshReceptors, Immunologicen
dc.subject.meshArabidopsis Proteinsen
dc.subject.meshTranscription Factorsen
dc.subject.meshGene Expressionen
dc.subject.meshEpigenesis, Geneticen
dc.subject.meshGene Expression Regulation, Planten
dc.subject.meshGenes, Planten
dc.subject.meshJumonji Domain-Containing Histone Demethylasesen
dc.subject.meshProtein Domainsen
dc.subject.meshNLR Proteinsen
dc.subject.meshPHD Zinc Fingersen
dc.titleThe Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expressionen
dc.title.serialPLOS Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2020-07-14en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen
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