BAK1 Mediates Light Intensity to Phosphorylate and Activate Catalases to Regulate Plant Growth and Development

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dc.contributor.authorZhang, Shanen
dc.contributor.authorLi, Chengen
dc.contributor.authorRen, Haihuaen
dc.contributor.authorZhao, Tongen
dc.contributor.authorLi, Qien
dc.contributor.authorWang, Shufenen
dc.contributor.authorZhang, Yanfengen
dc.contributor.authorXiao, Fangmingen
dc.contributor.authorWang, Xiaofengen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-03-02T13:29:33Zen
dc.date.available2020-03-02T13:29:33Zen
dc.date.issued2020-02-20en
dc.date.updated2020-03-02T12:41:49Zen
dc.description.abstractBAK1 (brassinosteroid-insensitive 1 (BRI1) associated receptor kinase 1) plays major roles in multiple signaling pathways as a coreceptor to regulate plant growth and development and stress response. However, the role of BAK1 in high light signaling is still poorly understood. Here we observed that overexpression of BAK1 in Arabidopsis interferes with the function of high light in promoting plant growth and development, which is independent of the brassinosteroid (BR) signaling pathway. Further investigation shows that high light enhances the phosphorylation of BAK1 and catalase activity, thereby reducing hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) accumulation. Catalase3 (CAT3) is identified as a BAK1-interacting protein by affinity purification and LC-MS/MS analysis. Biochemical analysis confirms that BAK1 interacts with and phosphorylates all three catalases (CAT1, CAT2, and CAT3) of <i>the</i> Arabidopsis genome, and the trans-phosphorylation sites of three catalases with BAK1-CD are identified by LC-MS/MS in vitro. Genetic analyses reveal that the BAK1 overexpression plants knocked out all the three <i>CAT</i> genes completely abolishing the effect of BAK1 on suppression of high light-promoted growth. This study first unravels the role of BAK1 in mediating high light-triggered activation of CATs, thereby degrading H<sub>2</sub>O<sub>2</sub> and regulating plant growth and development in Arabidopsis.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationZhang, S.; Li, C.; Ren, H.; Zhao, T.; Li, Q.; Wang, S.; Zhang, Y.; Xiao, F.; Wang, X. BAK1 Mediates Light Intensity to Phosphorylate and Activate Catalases to Regulate Plant Growth and Development. Int. J. Mol. Sci. 2020, 21, 1437.en
dc.identifier.doihttps://doi.org/10.3390/ijms21041437en
dc.identifier.urihttp://hdl.handle.net/10919/97104en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBAK1en
dc.subjecthigh lighten
dc.subjectCatalaseen
dc.subjectreceptor-like kinaseen
dc.subjectsignal transductionen
dc.titleBAK1 Mediates Light Intensity to Phosphorylate and Activate Catalases to Regulate Plant Growth and Developmenten
dc.title.serialInternational Journal of Molecular Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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