Changes with age in density of goblet cells in the small intestine of broiler chicks

dc.contributor.authorReynolds, K. L.en
dc.contributor.authorCloft, Sara E.en
dc.contributor.authorWong, Eric A.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2021-09-24T16:48:20Zen
dc.date.available2021-09-24T16:48:20Zen
dc.date.issued2020-05-01en
dc.date.updated2021-09-24T16:48:17Zen
dc.description.abstractGoblet cells secrete mucin 2 (Muc2), which is a major component of the mucus that lines the intestinal tract and creates a protective barrier between pathogens and the intestinal epithelial cells and thus are important for chick health. The objectives of this study were to determine the age-specific and intestinal segment–specific expression of Muc2 mRNA and changes in the number of goblet cells from late embryogenesis to early after hatch. Small intestinal samples from the duodenum, jejunum, and ileum were collected from Cobb 500 broilers at embryonic day 19 (e19), day of hatch (doh), and day 2 and 4 after hatch. Cells expressing Muc2 mRNA and mucin glycoprotein were detected by in situ hybridization or alcian blue and periodic acid–Schiff staining, respectively. Along the villi, there were many more cells expressing Muc2 mRNA than those stained for mucin glycoprotein. In the crypt, cells expressing Muc2 mRNA did not stain for mucin glycoprotein. There was an increase in the density of goblet cells in the villi and Muc2 mRNA expressing cells in the crypts of the jejunum and ileum from e19 to doh and day 2 to day 4, with no change between doh and day 2. In contrast, in the duodenum, the density of goblet cells in the villi and Muc2 mRNA expressing cells in the crypts remained constant from e19 to day 4. At day 4, the villi in the ileum had a greater density of goblet cells than the duodenum. In the crypt, the ileum had a greater density of Muc2 mRNA expressing cells than the duodenum at doh, and the ileum and jejunum both had greater densities of Muc2 mRNA expressing cells than the duodenum at day 4. These results indicate that the population of goblet cells has reached a steady state by doh in the duodenum, whereas in the jejunum and ileum, a steady-state population was not reached until after hatch.en
dc.description.versionPublished versionen
dc.format.extentPages 2342-2348en
dc.format.extent7 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.psj.2019.12.052en
dc.identifier.eissn1525-3171en
dc.identifier.issn0032-5791en
dc.identifier.issue5en
dc.identifier.orcidWong, Eric [0000-0002-1891-5466]en
dc.identifier.otherS0032-5791(20)30069-9 (PII)en
dc.identifier.pmid32359569en
dc.identifier.urihttp://hdl.handle.net/10919/105058en
dc.identifier.volume99en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000532702800005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectAgriculture, Dairy & Animal Scienceen
dc.subjectAgricultureen
dc.subjectgoblet cellen
dc.subjectmucin 2en
dc.subjectvillusen
dc.subjectcrypten
dc.subjectintestineen
dc.subjectGENE-EXPRESSIONen
dc.subjectMESSENGER-RNAen
dc.subjectIDENTIFICATIONen
dc.subjectMUCUSen
dc.subjectcrypten
dc.subjectgoblet cellen
dc.subjectintestineen
dc.subjectmucin 2en
dc.subjectvillusen
dc.subjectDairy & Animal Scienceen
dc.subject0605 Microbiologyen
dc.subject0702 Animal Productionen
dc.subject0908 Food Sciencesen
dc.subject.meshGoblet Cellsen
dc.subject.meshIntestine, Smallen
dc.subject.meshChick Embryoen
dc.subject.meshAnimalsen
dc.subject.meshChickensen
dc.subject.meshAvian Proteinsen
dc.subject.meshRNA, Messengeren
dc.subject.meshStaining and Labelingen
dc.subject.meshIn Situ Hybridizationen
dc.subject.meshAge Factorsen
dc.subject.meshMucin-2en
dc.subject.meshReal-Time Polymerase Chain Reactionen
dc.titleChanges with age in density of goblet cells in the small intestine of broiler chicksen
dc.title.serialPoultry Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2019-12-25en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
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