A mycotoxin transporter (4D) from a library of deoxynivalenol-tolerant microorganisms

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dc.contributor.authorJimenez-Sanchez, Celiaen
dc.contributor.authorWilson, Nina M.en
dc.contributor.authorMcMaster, Nicole J.en
dc.contributor.authorGantulga, Dashen
dc.contributor.authorFreedman, Benjamin G.en
dc.contributor.authorSenger, Ryan S.en
dc.contributor.authorSchmale, David G. IIIen
dc.contributor.departmentBiological Systems Engineeringen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2021-09-27T14:49:34Zen
dc.date.available2021-09-27T14:49:34Zen
dc.date.issued2020-03-01en
dc.date.updated2021-09-27T14:49:32Zen
dc.description.abstractNew strategies are needed to mitigate the mycotoxin deoxynivalenol (DON) in feed and food products. Microbial DNA fragments were generated from a library of DON-tolerant microorganisms. These fragments were screened in DON-sensitive yeast strains for their ability to modify or transport DON. Fragments were cloned into a PCR8/TOPO vector, and recombined into the yeast vector, pYES-DEST52. Resulting yeast transformants were screened in the presence of 100 ppm DON. Transformants that were able to grow in the presence of DON were plated on a selective medium, and the cloned microbial DNA fragments were sequenced. BLAST queries of one microbial DNA fragment (4D) showed a high degree of similarity to an ABC transporter. A series of screening and inhibition assays were conducted with a transport inhibitor (propanol), to test the hypothesis that 4D is a mycotoxin transporter. DON concentrations did not change for yeast transformants expressing 4D. The ability of yeast transformants expressing 4D to transport DON was inhibited by the addition of propanol. Moreover, yeast transformants expressing a known efflux pump (PDR5) showed similar trends in propanol transport inhibition compared to 4D. Future work should consider mycotoxin transporters such as 4D to the development of transgenic plants to limit DON accumulation in seeds.en
dc.description.versionPublished versionen
dc.format.extentPages 100023en
dc.format.mimetypeapplication/pdfen
dc.identifier100023 (Article number)en
dc.identifier.doihttps://doi.org/10.1016/j.toxcx.2020.100023en
dc.identifier.eissn2590-1710en
dc.identifier.issn2590-1710en
dc.identifier.orcidSenger, Ryan [0000-0002-2450-6693]en
dc.identifier.orcidSchmale, David [0000-0002-7003-7429]en
dc.identifier.otherPMC7286097en
dc.identifier.other100023 (PII)en
dc.identifier.pmid32550579en
dc.identifier.urihttp://hdl.handle.net/10919/105083en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/32550579en
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectDeoxynivalenolen
dc.subjectMicrobial DNA fragmenten
dc.subjectMycotoxinen
dc.subjectTransporteren
dc.subjectYeasten
dc.titleA mycotoxin transporter (4D) from a library of deoxynivalenol-tolerant microorganismsen
dc.title.serialToxicon: Xen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2020-01-10en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biological Systems Engineeringen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciences/Schmaleen

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