A study of the function of carotenoid pigments of a red psychrophilic micrococcus from Antarctica

dc.contributor.authorMinogue, Lucy Lancasteren
dc.contributor.departmentMicrobiologyen
dc.date.accessioned2023-04-07T05:16:48Zen
dc.date.available2023-04-07T05:16:48Zen
dc.date.issued1972en
dc.description.abstractA red psychrophilic micrococcus (Strain VPI20A) which was isolated from Antarctic Dry Valley soils was used in this investigation to determine the culture's sensitivity to ultraviolet radiation (180 to 300 nm). The spectrum of the typically pigmented culture showed that absorption maxima occurred at 210, 260, 320, 370, 390, 445, 465, 495 and 530 nm, with major maxima at 465, 495 and 530 nm, indicating presence of lycopene or other related compounds. Spectra from a white phenotype which had carotenogenesis suppressed by diphenylamine indicated maxima only in the ultraviolet range (390 nm and below). Comparative sensitivities of red and white cells to ultraviolet radiation were determined with cultures which had been harvested in both log and stationary phases. In the stationary phase, both types of cells had similar mortality curves. The stationary phase cultures were more sensitive than those of the log phase. However, log phase cells had differing death curves, with pigmented cells exhibiting greater resistance to ultraviolet radiation than nonpigmented cells. Addition of amino acids to the growth medium and changes in incubation temperature did not enhance or decrease carotenogenesis, and ascorbate, fluoride, methylene blue, cyanide, azide and glutathione did not affect synthesis of carotenoids in the concentrations used.en
dc.description.degreeM.S.en
dc.format.extentv, 53 leavesen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/114380en
dc.language.isoenen
dc.publisherVirginia Polytechnic Institute and State Universityen
dc.relation.isformatofOCLC# 33413499en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1972.M58en
dc.titleA study of the function of carotenoid pigments of a red psychrophilic micrococcus from Antarcticaen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineMicrobiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameM.S.en

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