Rootstocks Overexpressing StNPR1 and StDREB1 Improve Osmotic Stress Tolerance of Wild-Type Scion in Transgrafted Tobacco Plants

dc.contributor.authorHezema, Yasmine S.en
dc.contributor.authorShukla, Mukund R.en
dc.contributor.authorGoel, Aloken
dc.contributor.authorAyyanath, Murali M.en
dc.contributor.authorSherif, Sherif M.en
dc.contributor.authorSaxena, Praveen K.en
dc.contributor.departmentAlson H. Smith Jr. Agricultural Research and Extension Centeren
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2021-08-09T16:53:35Zen
dc.date.available2021-08-09T16:53:35Zen
dc.date.issued2021-08-05en
dc.date.updated2021-08-06T15:21:02Zen
dc.description.abstractIn grafted plants, the movement of long-distance signals from rootstocks can modulate the development and function of the scion. To understand the mechanisms by which tolerant rootstocks improve scion responses to osmotic stress (OS) conditions, mRNA transport of osmotic responsive genes (ORGs) was evaluated in a tomato/potato heterograft system. In this system, <i>Solanum tuberosum</i> was used as a rootstock and <i>Solanum lycopersicum</i> as a scion. We detected changes in the gene expression levels of 13 out of the 21 ORGs tested in the osmotically stressed plants; of these, only <i>NPR1</i> transcripts were transported across the graft union under both normal and OS conditions. Importantly, OS increased the abundance of <i>St</i><i>NPR1</i> transcripts in the tomato scion. To examine mRNA mobility in transgrafted plants, <i>StNPR1</i> and <i>StDREB1</i> genes representing the mobile and non-mobile transcripts, respectively, were overexpressed in tobacco (<i>Nicotiana tabacum</i>). The evaluation of transgenic tobacco plants indicated that overexpression of these genes enhanced the growth and improved the physiological status of transgenic plants growing under OS conditions induced by NaCl, mannitol and polyethylene glycol (PEG). We also found that transgenic tobacco rootstocks increased the OS tolerance of the WT-scion. Indeed, WT scions on transgenic rootstocks had higher ORGs transcript levels than their counterparts on non-transgenic rootstocks. However, neither <i>StNPR1</i> nor <i>StDREB1</i> transcripts were transported from the transgenic rootstock to the wild-type (WT) tobacco scion, suggesting that other long-distance signals downstream these transgenes could have moved across the graft union leading to OS tolerance. Overall, our results signify the importance of <i>StNPR1</i> and <i>StDREB1</i> as two anticipated candidates for the development of stress-resilient crops through transgrafting technology.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationHezema, Y.S.; Shukla, M.R.; Goel, A.; Ayyanath, M.M.; Sherif, S.M.; Saxena, P.K. Rootstocks Overexpressing StNPR1 and StDREB1 Improve Osmotic Stress Tolerance of Wild-Type Scion in Transgrafted Tobacco Plants. Int. J. Mol. Sci. 2021, 22, 8398.en
dc.identifier.doihttps://doi.org/10.3390/ijms22168398en
dc.identifier.urihttp://hdl.handle.net/10919/104607en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjecttransgraftingen
dc.subjectmRNA transporten
dc.subjectosmotic stressen
dc.subjectABAen
dc.subjectStNPR1en
dc.subjectStDREB1en
dc.subjectORGsen
dc.subjectROSen
dc.titleRootstocks Overexpressing StNPR1 and StDREB1 Improve Osmotic Stress Tolerance of Wild-Type Scion in Transgrafted Tobacco Plantsen
dc.title.serialInternational Journal of Molecular Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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