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Oxygen toxicity in Campylobacter jejuni: physiological comparison of a microaerophilic wild-type strain with an aerotolerant mutant

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dc.contributor.authorVercellone, Pamela Annen
dc.contributor.committeechairKrieg, Noel R.en
dc.contributor.committeememberSmibert, Robert M.en
dc.contributor.committeememberGregory, Eugene M.en
dc.contributor.departmentMicrobiologyen
dc.date.accessioned2014-03-14T21:50:28Zen
dc.date.adate2012-11-21en
dc.date.available2014-03-14T21:50:28Zen
dc.date.issued1988-12-05en
dc.date.rdate2012-11-21en
dc.date.sdate2012-11-21en
dc.description.abstractA comparative study of the microaerophilic <u>Campylobacter jejuni</u> strain H840 with an aerotolerant mutant, MC711-01, revealed that catalase and, to a lesser extent, SOD activity is correlated with the aerotolerance and enhanced resistance of MC7ll-0l to H₂O₂. When cells were cultured under 6% oxygen, the specific activity of catalase was significantly higher in crude extracts of MC711-01 than of H840. The catalase activity of MC711-01 more than doubled when cells were cultured under 21% oxygen, and this activity was 2.6 times greater than that of H84O; no corresponding increase was observed in strain H840. There was no significant difference in the mean SOD activity of the two strains when cultured under 6% oxygen or in H840 cells cultured under either 6% or 21% oxygen; however, the SOD activity of MC711-01 increased 1.5 times when cells were cultured under 21% oxygen. Survival studies revealed that MC711-01 was significantly more E resistant to H₂O₂ when cultured under either 6% or 21% oxygen. However, both MC711-01 and H84O were more susceptible to H₂O₂ when grown under 21% oxygen, indicating that both strains might be more highly stressed when the cells are grown at this oxygen tension. The present study suggests that in <u>C. jejuni</u>, the level of catalase activity may influence the degree of susceptibility to H₂O₂ and consequently, the degree of aerotolerance.en
dc.description.degreeMaster of Scienceen
dc.format.extentix, 70 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-11212012-040236en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11212012-040236/en
dc.identifier.urihttp://hdl.handle.net/10919/45965en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1988.V473.pdfen
dc.relation.isformatofOCLC# 19611406en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1988.V473en
dc.subject.lcshCampylobacteren
dc.subject.lcshOxygen -- Toxicologyen
dc.subject.lcshSpirillaceaeen
dc.titleOxygen toxicity in Campylobacter jejuni: physiological comparison of a microaerophilic wild-type strain with an aerotolerant mutanten
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineMicrobiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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