Detection of the fluorescing group of Pseudomonas by enzyme-linked immunosorbent assay (ELISA) for the prediction of shelf-life of dairy products

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dc.contributor.authorDishart, Katy Johannaen
dc.contributor.committeechairBishop, J. Russellen
dc.contributor.committeememberEigel, William N. IIIen
dc.contributor.committeememberYousten, Allan A.en
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2014-03-14T21:42:10Zen
dc.date.adate2009-08-04en
dc.date.available2014-03-14T21:42:10Zen
dc.date.issued1991-11-05en
dc.date.rdate2009-08-04en
dc.date.sdate2009-08-04en
dc.description.abstractAn enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies has been developed for the detection of the fluorescing group of Pseudomonas. The assay was used as a rapid test (6h) for predicting the shelf-life of pasteurized fluid milk. Milk samples were held at 7°C and tested weekly until determined to be unacceptable by daily sensory evaluation. Sterile milk samples were inoculated with target concentrations of 0 (control), 100, and 1000 cells/ml of Pseudomonas fluorescens on day 0. Samples were tested before and after preliminary incubations. Preliminary incubations conducted include milk alone and milk with broth (1:1) for 18h at 21°C. ELISA and plate counts were performed before and after preliminary incubation to determine the number of pseudomonads present and the relationship between ELISA and plate counts. These numbers were correlated to the shelf-life of each sample, as determined by sensory evaluation. Samples undergoing a preliminary incubation with only milk gave the best correlation to shelf-life (R=0.86).en
dc.description.degreeMaster of Scienceen
dc.format.extent63 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-08042009-040322en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08042009-040322/en
dc.identifier.urihttp://hdl.handle.net/10919/44151en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1991.D573.pdfen
dc.relation.isformatofOCLC# 25484749en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1991.D573en
dc.subject.lcshDairy products -- Handlingen
dc.subject.lcshEnzyme-linked immunosorbent assayen
dc.subject.lcshFood -- Shelf-life datingen
dc.subject.lcshPseudomonasen
dc.titleDetection of the fluorescing group of Pseudomonas by enzyme-linked immunosorbent assay (ELISA) for the prediction of shelf-life of dairy productsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineFood Science and Technologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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