Overexpression of ovine insulin-like growth factor-I (IGF-I) in the mammary glands of transgenic mice

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dc.contributor.authorWeber, Miriam S.en
dc.contributor.departmentDairy Scienceen
dc.date.accessioned2014-03-14T21:35:19Zen
dc.date.adate2009-05-02en
dc.date.available2014-03-14T21:35:19Zen
dc.date.issued1995en
dc.date.rdate2009-05-02en
dc.date.sdate2009-05-02en
dc.description.abstractEvidence indicates that the mammary gland may contribute to its own development by the production of insulin-like growth factor-I (IGF-I). To generate a model for investigation of the effects of enhanced IGF-I synthesis in the mammary gland, six transgenic founder mice were produced by microinjection of a cDNA sequence encoding ovine IGF-I (oIGF-I) under the control of the glucocorticoid-responsive mouse mammary tumor virus (MMTV) promoter. Transgenic lines were established from each MMTV-IGF-I transgenic founder, and four virgin and four lactating mice from each transgenic line were analyzed for transgene expression following administration of dexamethasone or vehicle. Virgin mice from three transgenic lines (15, 26, and 29) expressed oIGF-I mRNA and recombinant IGF-I in the mammary gland, although stimulation with exogenous dexamethasone was required for expression. Lactating mice from four transgenic lines (2, 15, 26, and 29) expressed oIGF-I mRNA in mammary tissue, but only three of those lines (15, 26, and 29) produced detectable recombinant IGF-I in milk and mammary tissue. Recombinant IGF-I was not detected in plasma from any of the transgenic lines. The biological activity of recombinant IGF-I secreted into the milk of lactating transgenic mice was demonstrated in vitro by stimulation of ([³H]thymidine incorporation into DNA of immortalized bovine mammary epithelial cells. The MMTV-IGF-I transgenic mice will provide a model for evaluating the effects of increased mammary synthesis of IGF-I on mammary gland development and function.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 60 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-05022009-040526en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05022009-040526/en
dc.identifier.urihttp://hdl.handle.net/10919/42399en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1995.W436.pdfen
dc.relation.isformatofOCLC# 34185504en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1995.W436en
dc.titleOverexpression of ovine insulin-like growth factor-I (IGF-I) in the mammary glands of transgenic miceen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineDairy Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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