Spatial Transcriptomics and Single-Nucleus Multi-Omics Analysis Revealing the Impact of High Maternal Folic Acid Supplementation on Offspring Brain Development

Simple item page
dc.contributor.authorXu, Xiguangen
dc.contributor.authorLin, Yuen
dc.contributor.authorYin, Liduoen
dc.contributor.authorSerpa, Priscila da Silvaen
dc.contributor.authorConacher, Benjaminen
dc.contributor.authorPacholec, Christinaen
dc.contributor.authorCarvallo, Franciscoen
dc.contributor.authorHrubec, Terryen
dc.contributor.authorFarris, Shannonen
dc.contributor.authorZimmerman, Kurten
dc.contributor.authorWang, Xiaobinen
dc.contributor.authorXie, Hehuangen
dc.date.accessioned2024-11-27T13:32:17Zen
dc.date.available2024-11-27T13:32:17Zen
dc.date.issued2024-11-07en
dc.date.updated2024-11-26T17:42:36Zen
dc.description.abstract<b>Background:</b> Folate, an essential vitamin B9, is crucial for diverse biological processes, including neurogenesis. Folic acid (FA) supplementation during pregnancy is a standard practice for preventing neural tube defects (NTDs). However, concerns are growing over the potential risks of excessive maternal FA intake. <b>Objectives/Methods:</b> Here, we employed a mouse model and spatial transcriptomic and single-nucleus multi-omics approaches to investigate the impact of high maternal FA supplementation during the periconceptional period on offspring brain development. <b>Results:</b> Maternal high FA supplementation affected gene pathways linked to neurogenesis and neuronal axon myelination across multiple brain regions, as well as gene expression alterations related to learning and memory in thalamic and ventricular regions. Single-nucleus multi-omics analysis revealed that maturing excitatory neurons in the dentate gyrus (DG) are particularly vulnerable to high maternal FA intake, leading to aberrant gene expressions and chromatin accessibility in pathways governing ribosomal biogenesis critical for synaptic formation. <b>Conclusions:</b> Our findings provide new insights into specific brain regions, cell types, gene expressions and pathways that can be affected by maternal high FA supplementation.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationXu, X.; Lin, Y.; Yin, L.; Serpa, P.S.; Conacher, B.; Pacholec, C.; Carvallo, F.; Hrubec, T.; Farris, S.; Zimmerman, K.; Wang, X.; Xie, H. Spatial Transcriptomics and Single-Nucleus Multi-Omics Analysis Revealing the Impact of High Maternal Folic Acid Supplementation on Offspring Brain Development. Nutrients 2024, 16, 3820.en
dc.identifier.doihttps://doi.org/10.3390/nu16223820en
dc.identifier.urihttps://hdl.handle.net/10919/123661en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleSpatial Transcriptomics and Single-Nucleus Multi-Omics Analysis Revealing the Impact of High Maternal Folic Acid Supplementation on Offspring Brain Developmenten
dc.title.serialNutrientsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nutrients-16-03820.pdf
Size:
4.34 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Journal Articles, Multidisciplinary Digital Publishing Institute (MDPI)
Scholarly Works, Biomedical Sciences and Pathobiology
Scholarly Works, Fralin Biomedical Research Institute at VTC
Scholarly Works, Fralin Life Sciences Institute
Scholarly Works, School of Neuroscience