Isolation and Structure Elucidation of Antiproliferative Agents From Madagascar Rainforests

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dc.contributor.authorKarkare, Sampada S.en
dc.contributor.committeechairKingston, David G. I.en
dc.contributor.committeememberBrewer, Karen J.en
dc.contributor.committeememberCarlier, Paul R.en
dc.contributor.departmentChemistryen
dc.date.accessioned2014-03-14T20:44:52Zen
dc.date.adate2007-10-31en
dc.date.available2014-03-14T20:44:52Zen
dc.date.issued2007-08-30en
dc.date.rdate2008-10-31en
dc.date.sdate2007-09-06en
dc.description.abstractThrough our continuing search for anticancer agents from Madagascar rainforests as a part of International Cooperative Biodiversity Group (ICBG), we received two extracts which were active against the A2780 human ovarian cancer cell line and hence were selected for further fractionation. Six compounds were isolated from these extracts. The structure elucidation and characterization of these compounds was carried out using mass spectrometry and 1D and 2D NMR techniques. The bioassay-guided fractionation of Roupellina (Strophanthus) boivinii yielded three new and one known cardenolide glycosides. The structure of the known cardenolide glycoside was determined after comparison of NMR data to that found in literature for digitoxigenin 3-O-β-D-glucopyranosyl-(1â 4)-α-L-acofriopyranoside. All four compounds exhibited good antiproliferative activity on the A2780 bioassay. The fractionation of the extract of Grewia sp. led to the isolation of one new and one known triterpenoid. The known triterpenoid was identified as 7β-hydroxy-23-deoxojessic acid and its structure was confirmed by comparison of its 1D and 2D NMR data to that found in literature.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-09062007-000547en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-09062007-000547/en
dc.identifier.urihttp://hdl.handle.net/10919/34945en
dc.publisherVirginia Techen
dc.relation.haspartSupporting_information_chap_2.pdfen
dc.relation.haspartAbbreviations.pdfen
dc.relation.haspartThesis-chap_1.pdfen
dc.relation.haspartAcknowledgements.pdfen
dc.relation.haspartThesis-chap_4.pdfen
dc.relation.haspartThesis-chap_2.pdfen
dc.relation.haspartFigures.pdfen
dc.relation.haspartTables.pdfen
dc.relation.haspartContents.pdfen
dc.relation.haspartSchemes.pdfen
dc.relation.haspartTitle_abstract.pdfen
dc.relation.haspartThesis-chap_5.pdfen
dc.relation.haspartSupporting_information_chap_3.pdfen
dc.relation.haspartThesis-chap_3.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectStrophanthusen
dc.subjecttriterpenoidsen
dc.subjectGrewia sp.en
dc.subjectRoupellinaen
dc.subjectGrewiaen
dc.subjectboivinideen
dc.subjectcardenolide glycosidesen
dc.subjectA2780en
dc.subjectnatural productsen
dc.titleIsolation and Structure Elucidation of Antiproliferative Agents From Madagascar Rainforestsen
dc.typeThesisen
thesis.degree.disciplineChemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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