Acute infection with Brachyspira hyodysenteriae affects mucin expression, glycosylation, and fecal MUC5AC

dc.contributor.authorLin, S.J.H.en
dc.contributor.authorHelm, E.T.en
dc.contributor.authorGabler, N.K.en
dc.contributor.authorBurrough, E.R.en
dc.date.accessioned2024-01-09T16:22:59Zen
dc.date.available2024-01-09T16:22:59Zen
dc.date.issued2023-01-06en
dc.description.abstractIntroduction: Infection with strongly β-hemolytic strains of Brachyspira hyodysenteriae leads to swine dysentery (SD), a production-limiting disease that causes mucohemorrhagic diarrhea and typhlocolitis in pigs. This pathogen has strong chemotactic activity toward mucin, and infected pigs often have a disorganized mucus layer and marked de novo expression of MUC5AC, which is not constitutively expressed in the colon. It has been shown that fucose is chemoattractant for B. hyodysenteriae, and a highly fermentable fiber diet can mitigate and delay the onset of SD. Methods: We used lectins targeting sialic acids in α-2,6 or α-2,3 linkages, N-acetylglucosamine (GlcNAc), α-linked L-fucose, and an immunohistochemical stain targeting N-glycolylneuraminic acid (NeuGc) to investigate the local expression of these mucin glycans in colonic tissues of pigs with acute SD. We used a commercial enzyme-linked immunosorbent assay (ELISA) to quantify fecal MUC5AC in infected pigs and assess its potential as a diagnostic monitoring tool and RNA in situ hybridization to detect IL-17A in the colonic mucosa. Results: Colonic mucin glycosylation during SD has an overall increase in fucose, a spatially different distribution of GlcNAc with more expression within the crypt lumens of the upper colonic mucosa, and decreased expression or a decreased trend of sialic acids in α-2,6 or α-2,3 linkages, and NeuGc compared to the controls. The degree of increased fucosylation was less in the colonic mucosa of pigs with SD and fed the highly fermentable fiber diet. There was a significant increase in MUC5AC in fecal and colonic samples of pigs with SD at the endpoint compared to the controls, but the predictive value for disease progression was limited. Discussion: Fucosylation and the impact of dietary fiber may play important roles in the pathogenesis of SD. The lack of predictive value for fecal MUC5AC quantification by ELISA is possibly due to the presence of other non-colonic sources of MUC5AC in the feces. The moderate correlation between IL-17A, neutrophils and MUC5AC confirms its immunoregulatory and mucin stimulatory role. Our study characterizes local alteration of mucin glycosylation in the colonic mucosa of pigs with SD after B. hyodysenteriae infection and may provide insight into host-pathogen interaction.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fcimb.2022.1042815en
dc.identifier.eissn2235-2988en
dc.identifier.issn2235-2988en
dc.identifier.orcidHelm, Emma [0000-0002-8025-9690]en
dc.identifier.pmid36683692en
dc.identifier.urihttps://hdl.handle.net/10919/117325en
dc.identifier.volume12en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/36683692en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBrachyspiraen
dc.subjectGlcNAcen
dc.subjectMUC2en
dc.subjectMUC5ACen
dc.subjectfucoseen
dc.subjectglycosylationen
dc.subjectsialic aciden
dc.subjectswine dysenteryen
dc.subject.meshFecesen
dc.subject.meshAnimalsen
dc.subject.meshSwineen
dc.subject.meshSpirochaetales Infectionsen
dc.subject.meshSwine Diseasesen
dc.subject.meshSialic Acidsen
dc.subject.meshFucoseen
dc.subject.meshInterleukin-17en
dc.subject.meshGlycosylationen
dc.subject.meshHost-Parasite Interactionsen
dc.subject.meshBrachyspira hyodysenteriaeen
dc.subject.meshMucin 5ACen
dc.titleAcute infection with Brachyspira hyodysenteriae affects mucin expression, glycosylation, and fecal MUC5ACen
dc.title.serialFrontiers in Cellular and Infection Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2022-11-28en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Animal Sciencesen
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