Fine Mapping and Candidate Gene Discovery at the Rsv3 Locus

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dc.contributor.authorBowman, Brian Carteren
dc.contributor.committeechairSaghai-Maroof, Mohammad A.en
dc.contributor.committeememberTyler, Brett M.en
dc.contributor.committeememberVeilleux, Richard E.en
dc.contributor.committeememberTolin, Sue A.en
dc.contributor.departmentCrop and Soil Environmental Sciencesen
dc.date.accessioned2017-04-04T19:49:11Zen
dc.date.adate2011-06-08en
dc.date.available2017-04-04T19:49:11Zen
dc.date.issued2011-04-28en
dc.date.rdate2016-09-27en
dc.date.sdate2011-05-18en
dc.description.abstractSoybean mosaic virus (SMV) is the most common member of the viral genus Potyvirus to infect soybeans (Glycine max [L.] Merr.) worldwide. SMV has been traditionally controlled by the deployment of single dominant, strain specific resistance genes, referred to as Rsv genes. Rsv1 is the most widely used form of SMV resistance with nine different alleles conferring resistance only to the lower numbered less virulent strains, G1 to G3. Rsv3 gives resistance to higher numbered more virulent strains G5 to G7. Soybean lines containing Rsv4, are resistant to all seven currently recognized North American SMV strains. In this study, the recently released soybean whole genome sequence was used to design molecular markers for fine mapping Rsv3 to a ~150 kb genomic region containing four coiled-coil nucleotide-binding leucine-rich repeat proteins. In a related study a large population segregating at the Rsv3 locus was screened for resistance to facilitate future characterization of this region. The markers identified in this study will allow for more accurate marker-assisted selection of Rsv3.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05182011-113247en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05182011-113247/en
dc.identifier.urihttp://hdl.handle.net/10919/76780en
dc.language.isoen_USen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectmolecular markeren
dc.subjectmicrosatelliteen
dc.subjectFine mappingen
dc.subjectLeucine rich repeaten
dc.subjectWhole genome sequenceen
dc.subjectSoybean mosaic virusen
dc.subjectSingle nucleotide polymorphismen
dc.titleFine Mapping and Candidate Gene Discovery at the Rsv3 Locusen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineCrop and Soil Environmental Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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