Development of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seeds

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dc.contributor.authorRosso, M. Lucianaen
dc.contributor.authorShang, Chaoen
dc.contributor.authorSong, Qijianen
dc.contributor.authorEscamilla, Diana M.en
dc.contributor.authorGillenwater, Jayen
dc.contributor.authorZhang, Boen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2021-03-15T11:42:35Zen
dc.date.available2021-03-15T11:42:35Zen
dc.date.issued2021-03-06en
dc.date.updated2021-03-12T14:40:30Zen
dc.description.abstractTrypsin inhibitors (TI), a common anti-nutritional factor in soybean, prevent animals’ protein digestibility reducing animal growth performance. No commercial soybean cultivars with low or null concentration of TI are available. The availability of a high throughput genotyping assay will be beneficial to incorporate the low TI trait into elite breeding lines. The aim of this study is to develop and validate a breeder friendly Kompetitive Allele Specific PCR (KASP) assay linked to low Kunitz trypsin inhibitor (KTI) in soybean seeds. A total of 200 F<sub>3:5</sub> lines derived from PI 547656 (low KTI) X Glenn (normal KTI) were genotyped using the BARCSoySNP6K_v2 Beadchip. F<sub>3:4</sub> and F<sub>3:5</sub> lines were grown in Blacksburg and Orange, Virginia in three years, respectively, and were measured for KTI content using a quantitative HPLC method. We identified three SNP markers tightly linked to the major QTL associated to low KTI in the mapping population. Based on these SNPs, we developed and validated the KASP assays in a set of 93 diverse germplasm accessions. The marker Gm08_44814503 has 86% selection efficiency for the accessions with low KTI and could be used in marker assisted breeding to facilitate the incorporation of low KTI content in soybean seeds.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRosso, M.L.; Shang, C.; Song, Q.; Escamilla, D.; Gillenwater, J.; Zhang, B. Development of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seeds. Int. J. Mol. Sci. 2021, 22, 2675.en
dc.identifier.doihttps://doi.org/10.3390/ijms22052675en
dc.identifier.urihttp://hdl.handle.net/10919/102714en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectKunitz trypsin inhibitoren
dc.subjectsoybeanen
dc.subjectquantitative trait locien
dc.subjectanti-nutritional factoren
dc.subjectsingle nucleotide polymorphismen
dc.subjectKASPen
dc.titleDevelopment of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seedsen
dc.title.serialInternational Journal of Molecular Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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