VTechWorks staff will be away for the Thanksgiving holiday beginning at noon on Wednesday, November 27, through Friday, November 29. We will resume normal operations on Monday, December 2. Thank you for your patience.
 

Improving Disease Resistance for Shrimp Through Application of Probiotics in Feed

Simple item page
dc.contributor.authorChoi, Moonyoungen
dc.contributor.committeechairKuhn, David D.en
dc.contributor.committeememberNeilson, Andrew P.en
dc.contributor.committeememberPonder, Monica A.en
dc.contributor.committeememberSmith, Stephen A.en
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2016-12-04T07:00:22Zen
dc.date.available2016-12-04T07:00:22Zen
dc.date.issued2015-06-12en
dc.description.abstractDiseases affecting shrimp contribute to billions of dollars of economic loss yearly to the aquaculture industry. Recently, one of the primary causative agents of disease has been Vibrio parahaemolyticus; in 2009, a new strain causing Early Mortality Syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) in shrimp emerged. Shrimp losses attributed to pathogens can be greatly reduced through probiotic use, which are known to act as natural immune enhancers and promote pathogen resistance. However, research on probiotic treatment against EMS disease is lacking. The overall project goal was to improve intensive shrimp production through direct application of probiotics in aquaculture feeds. The value of probiotics for the shrimp industry was evaluated by (1) reducing severity or mortality of V. parahaemolyticus disease in shrimp, (2) qPCR confirmation of Bacillus spore germination in shrimp gut, and (3) probiotic effectiveness evaluation for improving disease resistance. The virulence of several Vibrio spp. strains was examined and it was concluded the V. parahaemolyticus strain identified as the causative agent of EMS was the most lethal; EMS-infected shrimp exhibited 100% mortality within 36-hours of feed inoculation. The number of bacterial cells added to feed directly correlated with pathogenicity and only cells, not filtrate, was capable of causing death. Probiotic strains were evaluated and it was concluded that probiotic strains A, A/B blend, and B were the best candidates for promoting disease resistance against EMS. This research will provide the shrimp farming industry with information vital to developing a means for reducing economic loss from Vibrio-infected shrimp.en
dc.description.degreeMaster of Science in Life Sciencesen
dc.format.mediumETDen
dc.identifier.othervt_gsexam:5285en
dc.identifier.urihttp://hdl.handle.net/10919/73557en
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectantibioticsen
dc.subjectEarly Mortality Syndromeen
dc.subjectLitopenaues vannameien
dc.subjectPacific White Shrimpen
dc.subjectprobioticsen
dc.titleImproving Disease Resistance for Shrimp Through Application of Probiotics in Feeden
dc.typeThesisen
thesis.degree.disciplineFood Science and Technologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Science in Life Sciencesen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Choi_M_T_2015.pdf
Size:
10.59 MB
Format:
Adobe Portable Document Format
Download

Collections

Masters Theses