Differentiating Vaccine-Related Fowl Cholera from Naturally Occurring Disease

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dc.contributor.authorHutcheson, Anna R.en
dc.contributor.authorThompson, Kaseyen
dc.contributor.authorMaurer, John J.en
dc.contributor.authorFerguson, Naolaen
dc.contributor.authorGrogan, Karenen
dc.contributor.authorRoney, Stephenen
dc.contributor.authorSeahorn, Harmonyen
dc.contributor.authorLobsinger, Chrisen
dc.contributor.authorLee, Margie D.en
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2021-03-05T14:58:25Zen
dc.date.available2021-03-05T14:58:25Zen
dc.date.issued2020-12en
dc.description.abstractVaccine-related fowl cholera must be considered when flock mortality increases after use of a live Pasteurella multocida vaccine product. All registered live vaccines serotype as Heddleston 3,4; however, in some regions this is also the most common scrotype of outbreak isolates in broiler breeders and turkeys. Therefore, serotyping may not be useful for diagnosing vaccine-related fowl cholera. This project sought to apply a vaccine-specific test to differentiate vaccine-related disease from naturally occurring outbreaks. Results indicate that vaccine strains were commonly isolated from broiler breeders exhibiting signs of fowl cholera postvaccination, but some of these isolates exhibited only serotype 4 antigenicity. The isolates' lipopolysaccharides, the target antigen for serotyping, contained compositional changes that may explain the varying serotype results and virulence of the commercial preparations. These results suggest that vaccine-related disease may be common in broiler breeders, and live commercial vaccine preparations need to be assessed for serotype and titer prior to use in order to reduce vaccine-related fowl cholera.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesAH performed most of the experiments with the outbreak isolates and vaccine preps; CL and HS performed the initial isolation, identification, and serotyping of the outbreak isolates; SR and KG provided clinical assessment of suspect flocks; NF provided guidance on PCR development; KT supervised laboratory experiments; ML and JM supervised the research project. In addition to the funding from the Poultry Diagnostic and Research Center for characterizing the outbreak strains and developing the PCR, the carbohydrate analysis was supported by the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, U.S. Department of Energy grant (DE-SC0015662) to Parastoo Azadi at the Complex Carbohydrate Research Center.en
dc.description.sponsorshipPoultry Diagnostic and Research Center; Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, U.S. Department of Energy grantUnited States Department of Energy (DOE) [DE-SC0015662]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1637/aviandiseases-D-20-00024en
dc.identifier.eissn1938-4351en
dc.identifier.issn0005-2086en
dc.identifier.issue4en
dc.identifier.pmid33347552en
dc.identifier.urihttp://hdl.handle.net/10919/102619en
dc.identifier.volume64en
dc.language.isoenen
dc.rightsPublic Domainen
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/en
dc.subjectPasteurella multocidaen
dc.subjectfowl choleraen
dc.subjectserotypeen
dc.subjectvaccineen
dc.subjectlipopolysaccharidesen
dc.subjectPCRen
dc.subjectlectinen
dc.titleDifferentiating Vaccine-Related Fowl Cholera from Naturally Occurring Diseaseen
dc.title.serialAvian Diseasesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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