Mi-1.2, an R gene for aphid resistance in tomato, has direct negative effects on a zoophytophagous biocontrol agent, Orius insidiosus

dc.contributor.authorPallipparambil, Godshen R.en
dc.contributor.authorSayler, Ronald J.en
dc.contributor.authorShapiro, Jeffrey P.en
dc.contributor.authorThomas, Jean M. G.en
dc.contributor.authorKring, Timothy J.en
dc.contributor.authorGoggin, Fiona L.en
dc.date.accessioned2016-06-22T14:28:55Zen
dc.date.available2016-06-22T14:28:55Zen
dc.date.issued2015-02en
dc.description.abstractMi-1.2 is a single dominant gene in tomato that confers race-specific resistance against certain phloem-feeding herbivores including aphids, whiteflies, psyllids, and root-knot nematodes. Few prior studies have considered the potential non-target effects of race-specific resistance genes (R genes), and this paper evaluates the compatibility of Mi-mediated resistance in tomato with a beneficial zoophytophagous predator, Orius insidiosus (Say). In addition to preying on aphids and other pests, this piercing-sucking insect also feeds from the xylem, epidermis, and/or mesophyll, and oviposits within plant tissues. Comparison of O. insidiosus confined to isogenic tomato plants with and without Mi-1.2 revealed that immatures of O. insidiosus had lower survival on resistant plants even when the immatures were provisioned with prey that did not feed on the host plant. Molecular gut content analysis confirmed that adults and immatures of O. insidiosus feed on both resistant (Mi-1.2+) and susceptible (Mi-1.2-) genotypes, and bioassays suggest that resistance does not affect oviposition rates, plant sampling, or prey acceptance by O. insidiosus adults. These results demonstrate a direct negative impact of R-gene-mediated host plant resistance on a non-target beneficial species, and reveal that Mi-mediated resistance can impact organisms that do not feed on phloem sap. Through laser capture microdissection and RT-PCR, Mi-1.2 transcripts were detected in the epidermis and mesophyll as well as the phloem of tomato plants, consistent with our observations that Mi-mediated resistance is active outside the phloem. These results suggest that the mode of action and potential ecological impacts of Mi-mediated resistance are broader than previously assumed.en
dc.description.versionPublished versionen
dc.format.extent549 - 557 page(s)en
dc.identifier.doihttps://doi.org/10.1093/jxb/eru361en
dc.identifier.eissn1460-2431en
dc.identifier.issue2en
dc.identifier.urihttp://hdl.handle.net/10919/71401en
dc.identifier.volume66en
dc.language.isoenen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/25189594en
dc.rightsCreative Commons Attribution 3.0 Unporteden
dc.rights.holderThe Author(s)en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectBiological controlen
dc.subjectMi-1en
dc.subjectMi-1.2en
dc.subjectR geneen
dc.subjecthost plant resistanceen
dc.subjectinsect resistanceen
dc.subjectintegrated pest managementen
dc.subjectmesophyll feedingen
dc.subjectnatural enemyen
dc.subjectplant defenceen
dc.subjectxylem feeding.en
dc.subjectAnimalsen
dc.subjectAphidsen
dc.subjectDigestive Systemen
dc.subjectDisease Resistanceen
dc.subjectFemaleen
dc.subjectGene Expression Regulation, Planten
dc.subjectGenes, Planten
dc.subjectHerbivoryen
dc.subjectHeteropteraen
dc.subjectLycopersicon esculentumen
dc.subjectOvipositionen
dc.subjectPest Control, Biologicalen
dc.subjectPlant Diseasesen
dc.subjectRNA, Messengeren
dc.subjectSurvival Analysisen
dc.titleMi-1.2, an R gene for aphid resistance in tomato, has direct negative effects on a zoophytophagous biocontrol agent, Orius insidiosusen
dc.title.serialJournal of Experimental Botanyen
dc.typeArticle - Refereeden
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
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