Imaginal Discs - A New Source of Chromosomes for Genome Mapping of the Yellow Fever Mosquito Aedes aegypti

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dc.contributor.authorSharakhova, Maria V.en
dc.contributor.authorTimoshevskiy, Vladimir A.en
dc.contributor.authorYang, Fanen
dc.contributor.authorDemin, Sergei Iu.en
dc.contributor.authorSeverson, David W.en
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.departmentEntomologyen
dc.contributor.departmentStatisticsen
dc.date.accessioned2017-01-05T22:18:49Zen
dc.date.available2017-01-05T22:18:49Zen
dc.date.issued2011-10-01en
dc.description.abstractBackground: The mosquito Aedes aegypti is the primary global vector for dengue and yellow fever viruses. Sequencing of the Ae. aegypti genome has stimulated research in vector biology and insect genomics. However, the current genome assembly is highly fragmented with only ,31% of the genome being assigned to chromosomes. A lack of a reliable source of chromosomes for physical mapping has been a major impediment to improving the genome assembly of Ae. aegypti. Methodology/Principal Findings: In this study we demonstrate the utility of mitotic chromosomes from imaginal discs of 4th instar larva for cytogenetic studies of Ae. aegypti. High numbers of mitotic divisions on each slide preparation, large sizes, and reproducible banding patterns of the individual chromosomes simplify cytogenetic procedures. Based on the banding structure of the chromosomes, we have developed idiograms for each of the three Ae. aegypti chromosomes and placed 10 BAC clones and a 18S rDNA probe to precise chromosomal positions. Conclusion: The study identified imaginal discs of 4th instar larva as a superior source of mitotic chromosomes for Ae. aegypti. The proposed approach allows precise mapping of DNA probes to the chromosomal positions and can be utilized for obtaining a high-quality genome assembly of the yellow fever mosquito.en
dc.description.versionPublished versionen
dc.format.extent? - ? (9) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0001335en
dc.identifier.issn1935-2727en
dc.identifier.issue10en
dc.identifier.urihttp://hdl.handle.net/10919/73968en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherPLOSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000296579700011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectInfectious Diseasesen
dc.subjectParasitologyen
dc.subjectTropical Medicineen
dc.subjectQUANTITATIVE TRAIT LOCIen
dc.subjectMALARIA PARASITE SUSCEPTIBILITYen
dc.subjectSALIVARY-GLAND CHROMOSOMESen
dc.subjectDROSOPHILA-MELANOGASTERen
dc.subjectANOPHELES-GAMBIAEen
dc.subjectLINKAGE MAPen
dc.subjectDENGUEen
dc.subjectHETEROCHROMATINen
dc.subjectSEQUENCEen
dc.subjectDISEASEen
dc.titleImaginal Discs - A New Source of Chromosomes for Genome Mapping of the Yellow Fever Mosquito Aedes aegyptien
dc.title.serialPLoS Neglected Tropical Diseasesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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