Bead size has a greater effect on in vitro elution from antimicrobial-impregnated calcium sulfate beads than drug concentration

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dc.contributor.authorOlsen, Ronald S.en
dc.contributor.authorSawyere, Dominique M.en
dc.contributor.authorDavis, Jennifer L.en
dc.contributor.authorLanz, Otto I.en
dc.contributor.authorWerre, Stephen R.en
dc.date.accessioned2023-08-03T14:39:25Zen
dc.date.available2023-08-03T14:39:25Zen
dc.date.issued2023-05en
dc.description.abstractOBJECTIVE To compare the elution characteristics of amikacin-impregnated calcium sulfate (CaSO4) beads based on different drug concentrations and bead size configurations. SAMPLE Six groups of amikacin-impregnated CaSO4 beads and one negative control group. PROCEDURES Amikacin-impregnated CaSO4 beads were formed with either 500 mg (low-concentration) or 1 g (high-concentration) of amikacin per 15 g CaSO4 hemihydrate powder. The number of beads necessary to approximate 150 mg of amikacin for each of the 3 bead sizes (3 mm, 5 mm, and 7 mm) at both low and high concentrations were placed in 6 mL of phosphate-buffered saline. The saline was sampled at 14 time points over 28 days. Amikacin concentrations were determined using liquid chromatography-mass spectrometry. RESULTS Smaller beads reached higher mean peak concentrations than larger beads (P < .0006). Peak concentrations for the low-and high-concentration groups were 20.5 mg/mL and 27.4 mg/mL, 13.1 mg/mL and 14.0 mg/mL, and 8.85 mg/mL and 6.75 mg/mL for the 3 mm, 5 mm, and 7 mm beads, respectively. Bead size also affected the length of therapeutic duration, lasting 6 days for the 3 mm and 5 mm beads and 9 days for the 7 mm beads. However, this was only statistically evident among the high-concentration beads (P < .044). Antimicrobial concentration within the same bead sizes did not affect elution. CLINICAL RELEVANCE Amikacin-impregnated CaSO4 beads achieved extreme supratherapeutic eluent concentrations. While additional studies are needed, bead size significantly affected elution with smaller beads reaching higher peak concentrations and 7 mm, high-concentration beads demonstrating a longer therapeutic duration than smaller beads.en
dc.description.notesThe authors thank the Veterinary Memorial Fund, Virginia -Maryland College of Veterinary Medicine for financial support of this project and Kerrier, LLC for providing Absorbable Bead Kits for this project. The authors declare that there were no conflicts of interest.en
dc.description.sponsorshipVeterinary Memorial Fund, Virginia -Maryland College of Veterinary Medicineen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.2460/ajvr.22.12.0216en
dc.identifier.eissn1943-5681en
dc.identifier.issn0002-9645en
dc.identifier.issue5en
dc.identifier.pmid36881500en
dc.identifier.urihttp://hdl.handle.net/10919/115975en
dc.identifier.volume84en
dc.language.isoenen
dc.publisherAmerican Veterinary Medical Associationen
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.titleBead size has a greater effect on in vitro elution from antimicrobial-impregnated calcium sulfate beads than drug concentrationen
dc.title.serialAmerican Journal of Veterinary Researchen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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