Photolysis of Staphyloxanthin in Methicillin-Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Species

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dc.contributor.authorDong, Pu-Tingen
dc.contributor.authorMohammad, Haroonen
dc.contributor.authorHui, Jieen
dc.contributor.authorLeanse, Leon G.en
dc.contributor.authorLi, Junjieen
dc.contributor.authorLiang, Lijiaen
dc.contributor.authorDai, Tianhongen
dc.contributor.authorSeleem, Mohamed N.en
dc.contributor.authorCheng, Ji-Xinen
dc.date.accessioned2020-09-21T16:14:00Zen
dc.date.available2020-09-21T16:14:00Zen
dc.date.issued2019-06-05en
dc.date.updated2020-09-21T16:13:55Zen
dc.description.abstractConfronted with the severe situation that the pace of resistance acquisition is faster than the clinical introduction of new antibiotics, health organizations are calling for effective approaches to combat methicillin-resistant Staphylococcus aureus (MRSA) infections. Here, an approach to treat MRSA through photolysis of staphyloxanthin, an antioxidant residing in the microdomain of S. aureus membrane, is reported. This photochemistry process is uncovered through transient absorption imaging and quantitated by absorption spectroscopy, Raman spectroscopy, and mass spectrometry. Photolysis of staphyloxanthin transiently elevates the membrane permeability and renders MRSA highly susceptible to hydrogen peroxide attack. Consequently, staphyloxanthin photolysis by low-level 460 nm light eradicates MRSA synergistically with hydrogen peroxide and other reactive oxygen species. The effectiveness of this synergistic therapy is well validated in MRSA planktonic culture, MRSA-infected macrophage cells, stationary-phase MRSA, persisters, S. aureus biofilms, and two mice wound infection models. Collectively, the work demonstrates that staphyloxanthin photolysis is a new therapeutic platform to treat MRSA infections.en
dc.description.versionPublished versionen
dc.format.extent12 page(s)en
dc.format.mediumElectronic-eCollectionen
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 1900030 (Article number)en
dc.identifier.doihttps://doi.org/10.1002/advs.201900030en
dc.identifier.eissn2198-3844en
dc.identifier.issn2198-3844en
dc.identifier.issue11en
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458]en
dc.identifier.otherADVS1026 (PII)en
dc.identifier.pmid31179216 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/100030en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherWileyen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectChemistry, Multidisciplinaryen
dc.subjectNanoscience & Nanotechnologyen
dc.subjectMaterials Science, Multidisciplinaryen
dc.subjectChemistryen
dc.subjectMaterials Scienceen
dc.subjectmethicillin-resistant Staphylococcus aureus (MRSA)en
dc.subjectphototherapyen
dc.subjectstaphyloxanthin photobleachingen
dc.subjecttransient absorption microscopyen
dc.subjectBIOSYNTHESISen
dc.subjectMICROBIOLOGYen
dc.subjectFLUORESCENCEen
dc.subjectCAROTENOIDSen
dc.subjectCOMBINATIONen
dc.subjectDAPTOMYCINen
dc.subjectDEPENDENCEen
dc.subjectVIRULENCEen
dc.subjectBIOFILMSen
dc.subjectDRUGSen
dc.subjectmethicillinā€resistant Staphylococcus aureus (MRSA)en
dc.titlePhotolysis of Staphyloxanthin in Methicillin-Resistant Staphylococcus aureus Potentiates Killing by Reactive Oxygen Speciesen
dc.title.serialAdvanced Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Techen

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